▼ Enzyme conversion An immobilised enzyme process is used in an ice-cream factory to hydrolyse lactose (C12H22O11) to glucose (C6H12O6) and galactose (C6H1206): C12H22O11 + H2O →→ C6H12O + C6H12O6 Gel beads containing ß-galactosidase are packed into a column reactor; 2500 kg of lactose enters the reactor per day as a 10% solution in water at 25°C. The reactor operates at steady state and 32°C; all of the lactose is converted. Because the heat of reaction for enzyme conversions is not as great as for cell culture, sensible heat changes and heats of mixing cannot be ignored. Lactose Water Ah (kJ gmol¹) C, (cal g¹ ºC-¹) Ahm (kcal gmol¹) 3.7 -5652.5 0.30 1.0 Glucose -2805.0 0.30 5.6 Galactose -2805.7 0.30 5.6 (a) What is the standard heat of reaction for this enzyme conversion? (b) Estimate the heating or cooling requirements for this process. State explicitly whether heating or cooling is needed.

Introduction to Chemical Engineering Thermodynamics
8th Edition
ISBN:9781259696527
Author:J.M. Smith Termodinamica en ingenieria quimica, Hendrick C Van Ness, Michael Abbott, Mark Swihart
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Chapter1: Introduction
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▼ Enzyme conversion
An immobilised enzyme process is used in an ice-cream factory to hydrolyse lactose (C12H22O11) to glucose (C6H12O6) and galactose (C6H1206):
C12H22O11 + H2O →→ C6H12O + C6H12O6
Gel beads containing ß-galactosidase are packed into a column reactor; 2500 kg of lactose enters the reactor per day as a 10% solution in water at
25°C. The reactor operates at steady state and 32°C; all of the lactose is converted. Because the heat of reaction for enzyme conversions is not as great
as for cell culture, sensible heat changes and heats of mixing cannot be ignored.
Lactose
Water
Ah (kJ gmol¹) C, (cal g¹ ºC-¹) Ahm (kcal gmol¹)
3.7
-5652.5
0.30
1.0
Glucose
-2805.0
0.30
5.6
Galactose
-2805.7
0.30
5.6
(a) What is the standard heat of reaction for this enzyme conversion?
(b) Estimate the heating or cooling requirements for this process. State explicitly whether heating or cooling is needed.
Transcribed Image Text:▼ Enzyme conversion An immobilised enzyme process is used in an ice-cream factory to hydrolyse lactose (C12H22O11) to glucose (C6H12O6) and galactose (C6H1206): C12H22O11 + H2O →→ C6H12O + C6H12O6 Gel beads containing ß-galactosidase are packed into a column reactor; 2500 kg of lactose enters the reactor per day as a 10% solution in water at 25°C. The reactor operates at steady state and 32°C; all of the lactose is converted. Because the heat of reaction for enzyme conversions is not as great as for cell culture, sensible heat changes and heats of mixing cannot be ignored. Lactose Water Ah (kJ gmol¹) C, (cal g¹ ºC-¹) Ahm (kcal gmol¹) 3.7 -5652.5 0.30 1.0 Glucose -2805.0 0.30 5.6 Galactose -2805.7 0.30 5.6 (a) What is the standard heat of reaction for this enzyme conversion? (b) Estimate the heating or cooling requirements for this process. State explicitly whether heating or cooling is needed.
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