Chapter 9, Problem 9.14P

(a)

Interpretation Introduction

Interpretation:

In the Eadie-Hofstee plot, the inhibition mechanism of line A is to be identified and represented in the plot.

Concept Introduction:

The graph that is obtained by plotting the substrate reaction rate $\left(-r_s\right)$ on Y-axis and the ratio of the substrate reaction rate to the substrate concentration $\left(\frac{-r_s}{S}\right)$ on X-axis is termed as an Eadie-Hofstee plot. The equation that represents the Eadie-Hofstee plot is

shown below.

  $-r_s=V_{\max }-K_M\left(\frac{-r_s}{S}\right)$

In this plot, the slope is represented by $-K_M$ and the intercept is $V_{\max }$.

(b)

Interpretation Introduction

Interpretation:

In the Eadie-Hofstee plot, the inhibition mechanism of line B is to be identified and represented in the plot.

Concept Introduction:

The graph that is obtained by plotting the substrate reaction rate $\left(-r_s\right)$ on Y-axis and the ratio of the substrate reaction rate to the substrate concentration $\left(\frac{-r_s}{S}\right)$ on X-axis is termed as an Eadie-Hofstee plot. The equation that represents the Eadie-Hofstee plot is shown below.

  $-r_s=V_{\max }-K_M\left(\frac{-r_s}{S}\right)$

In this plot, the slope is represented by $-K_M$ and the intercept is $V_{\max }$.

(c)

Interpretation Introduction

Interpretation:

In the Eadie-Hofstee plot, the inhibition mechanism of line C is to be identified and represented in the plot.

Concept Introduction:

The graph that is obtained by plotting the substrate reaction rate $\left(-r_s\right)$ on Y-axis and the ratio of the substrate reaction rate to the substrate concentration $\left(\frac{-r_s}{S}\right)$ on X-axis is termed as an Eadie-Hofstee plot. The equation that represents the Eadie-Hofstee plot is shown below.

  $-r_s=V_{\max }-K_M\left(\frac{-r_s}{S}\right)$

In this plot, the slope is represented by $-K_M$ and the intercept is $V_{\max }$.