Chapter 9, Problem 30P

Summary Introduction

To review:

Based on the DNA sequence encoding five amino acid polypeptide and answer the following:

1. Identify the template and coding strands of DNA and locate the sequence encoding five amino acid of the polypeptides.

2. Give the sequence and polarity of mRNA encoding the polypeptide.

3. Give the polypeptide sequence and identify the N- and C- terminus.

4. Identify the region encoding the shine dalgarno sequence.

5. Explain the function of Shine dalgarno sequence.

Introduction:

In molecular biology, the central dogma describes the formation of polypeptide chain from the template DNA strand through the intermediate formation of mRNA.

Transcription Translation

DNA —- ———–> mRNA – ———-> polypeptide

The DNA contains the information for the given polypeptide in the form of specific sequences of codons. The codon is the triplet of nitrogen bases coding the specific amino acids. These amino acids are bound together by peptide bond to form polypeptides.