Anatomy & Physiology: The Unity of Form and Function
8th Edition
ISBN: 9781259277726
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 8.5, Problem 15AYLO
Names and locations of the three foot arches
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 8 Solutions
Anatomy & Physiology: The Unity of Form and Function
Ch. 8.1 - Name the major components of the axial skeleton....Ch. 8.1 - Explain why an adult doesnot have asmany bones as...Ch. 8.1 - Prob. 3BYGOCh. 8.1 - The difference between the axial and appendicular...Ch. 8.1 - The typical number of named bones in an adult; why...Ch. 8.1 - Prob. 3AYLOCh. 8.1 - Names of the various outgrowths, depressions,...Ch. 8.2 - Name the paranasal sinuses and state their...Ch. 8.2 - Explain the difference Between a cranial bone and...Ch. 8.2 - Prob. 6BYGO
Ch. 8.2 - Prob. 7BYGOCh. 8.2 - Prob. 8BYGOCh. 8.2 - Prob. 1AYLOCh. 8.2 - Prob. 2AYLOCh. 8.2 - The collective function of the skull foramina; the...Ch. 8.2 - Major features of the cranium; the difference...Ch. 8.2 - Names of the six different cranial bones; which...Ch. 8.2 - The location and extent of the frontal bone; the...Ch. 8.2 - Prob. 7AYLOCh. 8.2 - Prob. 8AYLOCh. 8.2 - The location and extent of the occipital boor, its...Ch. 8.2 - The location and extent of the sphenoid bone; its...Ch. 8.2 - Prob. 11AYLOCh. 8.2 - Prob. 12AYLOCh. 8.2 - Prob. 13AYLOCh. 8.2 - Prob. 14AYLOCh. 8.2 - Prob. 15AYLOCh. 8.2 - Prob. 16AYLOCh. 8.2 - Prob. 17AYLOCh. 8.2 - Prob. 18AYLOCh. 8.2 - Prob. 19AYLOCh. 8.2 - Prob. 20AYLOCh. 8.2 - Prob. 21AYLOCh. 8.2 - Prob. 22AYLOCh. 8.2 - Prob. 23AYLOCh. 8.2 - Names and locations of the fontanelles of the...Ch. 8.3 - Prob. 9BYGOCh. 8.3 - Prob. 10BYGOCh. 8.3 - Prob. 11BYGOCh. 8.3 - Prob. 12BYGOCh. 8.3 - Distinguish between true, false, aid floating...Ch. 8.3 - Prob. 14BYGOCh. 8.3 - Prob. 1AYLOCh. 8.3 - Prob. 2AYLOCh. 8.3 - Features of a typical vertebraCh. 8.3 - The five classes of vertebrae and the number of...Ch. 8.3 - Prob. 5AYLOCh. 8.3 - Prob. 6AYLOCh. 8.3 - Prob. 7AYLOCh. 8.3 - Prob. 8AYLOCh. 8.3 - Prob. 9AYLOCh. 8.3 - Prob. 10AYLOCh. 8.3 - Prob. 11AYLOCh. 8.3 - Prob. 12AYLOCh. 8.3 - Prob. 13AYLOCh. 8.3 - Prob. 14AYLOCh. 8.3 - Which ribs differ from that typical anatomy, and...Ch. 8.3 - Prob. 16AYLOCh. 8.4 - Prob. 15BYGOCh. 8.4 - Prob. 16BYGOCh. 8.4 - What three bones meet at the elbow? Identify the...Ch. 8.4 - Prob. 18BYGOCh. 8.4 - Prob. 19BYGOCh. 8.4 - Names and locations of the 4 bones of the pectoral...Ch. 8.4 - Names of the joints at which the humerus...Ch. 8.4 - Features of the clavicle, including the sternal...Ch. 8.4 - Prob. 4AYLOCh. 8.4 - Prob. 5AYLOCh. 8.4 - Prob. 6AYLOCh. 8.4 - Prob. 7AYLOCh. 8.4 - Prob. 8AYLOCh. 8.4 - Prob. 9AYLOCh. 8.4 - Prob. 10AYLOCh. 8.5 - Prob. 20BYGOCh. 8.5 - Prob. 21BYGOCh. 8.5 - Prob. 22BYGOCh. 8.5 - Prob. 23BYGOCh. 8.5 - Prob. 24BYGOCh. 8.5 - Prob. 25BYGOCh. 8.5 - Prob. 1AYLOCh. 8.5 - Prob. 2AYLOCh. 8.5 - Prob. 3AYLOCh. 8.5 - Three childhood bones that fuse to form each adult...Ch. 8.5 - Prob. 5AYLOCh. 8.5 - Prob. 6AYLOCh. 8.5 - Names of the four regions of the lower limb and...Ch. 8.5 - Features of the femur, including the head, neck,...Ch. 8.5 - Prob. 9AYLOCh. 8.5 - Features of the tibia, including the lateral and...Ch. 8.5 - Prob. 11AYLOCh. 8.5 - Prob. 12AYLOCh. 8.5 - Prob. 13AYLOCh. 8.5 - Why the elbows and knees flex in opposite...Ch. 8.5 - Names and locations of the three foot archesCh. 8 - Prob. 1TYRCh. 8 - Prob. 2TYRCh. 8 - Prob. 3TYRCh. 8 - All of the following are groups of vertebrae...Ch. 8 - Prob. 5TYRCh. 8 - The tubercle of a rib articulates with a. the...Ch. 8 - The disc-shaped head of the radius articulates...Ch. 8 - Prob. 8TYRCh. 8 - Prob. 9TYRCh. 8 - Prob. 10TYRCh. 8 - Prob. 11TYRCh. 8 - The external auditory canal is a passage in the...Ch. 8 - Prob. 13TYRCh. 8 - The __________ bone has greater and lesser wings...Ch. 8 - Prob. 15TYRCh. 8 - Prob. 16TYRCh. 8 - Prob. 17TYRCh. 8 - Prob. 18TYRCh. 8 - Prob. 19TYRCh. 8 - Prob. 20TYRCh. 8 - Prob. 1BYMVCh. 8 - Prob. 2BYMVCh. 8 - Prob. 3BYMVCh. 8 - Prob. 4BYMVCh. 8 - Prob. 5BYMVCh. 8 - Prob. 6BYMVCh. 8 - Prob. 7BYMVCh. 8 - Prob. 8BYMVCh. 8 - Prob. 9BYMVCh. 8 - Prob. 10BYMVCh. 8 - Prob. 1WWTSCh. 8 - Prob. 2WWTSCh. 8 - Prob. 3WWTSCh. 8 - Prob. 4WWTSCh. 8 - Prob. 5WWTSCh. 8 - Prob. 6WWTSCh. 8 - Prob. 7WWTSCh. 8 - The most frequently broken bone is the humerus.Ch. 8 - Prob. 9WWTSCh. 8 - Sesamoid bones are found along the sutures between...Ch. 8 - Prob. 1TYCCh. 8 - Prob. 2TYCCh. 8 - Between any two of the unfused vertebrae (cervical...Ch. 8 - In adolescents, trauma sometimes separates the...Ch. 8 - Prob. 5TYC
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