To answer: Similarity between the results of Southern blot and DNA microarray. Introduction: DNA microarray is composed of single-stranded DNA molecules (ssDNA) as cDNA or genomic DNA, which are attached to the solid substrate such as glass slide, a nylon membrane, silicon chips, or other microarray substrates. It is also called DNA chip as it contains a collection of thousands of various DNA molecules at a specific site on the array. The single array may contain DNA molecules from one or more species. The labeled (tagged with fluorescent) DNA sample is washed over the DNA microarray and the DNA molecule binds to its complementary DNA sequence on the array. The presence of targeted DNA sequence is identified by the development of fluorescence. This technique is used to find DNA or RNA in a sample and to evaluate the expression of genes.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 4CT

Summary Introduction

To answer:

Similarity between the results of Southern blot and DNA microarray.

Introduction:

DNA microarray is composed of single-stranded DNA molecules (ssDNA) as cDNA or genomic DNA, which are attached to the solid substrate such as glass slide, a nylon membrane, silicon chips, or other microarray substrates. It is also called DNA chip as it contains a collection of thousands of various DNA molecules at a specific site on the array. The single array may contain DNA molecules from one or more species. The labeled (tagged with fluorescent) DNA sample is washed over the DNA microarray and the DNA molecule binds to its complementary DNA sequence on the array. The presence of targeted DNA sequence is identified by the development of fluorescence. This technique is used to find DNA or RNA in a sample and to evaluate the expression of genes.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Respond to the following in a minimum of 175 words: How might CRISPR-Cas 9 be used in research or, eventually, therapeutically in patients? What are some potential ethical issues associated with using this technology? Do the advantages of using this technology outweigh the disadvantages (or vice versa)? Explain your position.

You are studying the effect of directional selection on body height in three populations (graphs a, b, and c below). (a) What is the selection differential? Show your calculation. (2 pts) (b) Which population has the highest narrow sense heritability for height? Explain your answer. (2 pts) (c) If you examined the offspring in the next generation in each population, which population would have the highest mean height? Why? (2 pts) (a) Midoffspring height (average height of offspring) Short Short Short Short (c) Short (b) Short Tall Short Tall Short Short Tall Midparent height (average height of Mean of population = 65 inches Mean of breading parents = 70 inches Mean of population = 65 inches Mean of breading parents = 70 inches Mean of population = 65 inches Mean of breading parents = 70 inches

P You are studying a population of 100 flowers that has two alleles at a locus for flower color, blue (B) and green (G). There are 15 individuals with the BB genotype, 70 individuals with the BG genotype, and 15 individuals with the GG genotype. (a) What are the allele frequencies of B and G in the starting population? Show your calculations. (2 pts) (b) Is this population in Hardy-Weinberg equilibrium? Show your calculations. (3 pts) 12pt v Paragraph BIU UA AV & VT2V f CO V