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Chapter 8, Problem 4MC
Summary Introduction

Introduction:

The recombinant DNA technology involves isolation of genetic materials (DNA) from donor organisms (human, plant, or microorganism) and inserts them into recipient organisms after genetic manipulation. The main tools involved in the techniques are nucleic acids, restriction enzymes, ligase, vectors, reverse transcriptase, and mutagens. This technology is used to introduce genetically modified organism (GMO). The main goals of this technology are to provide (i) elimination of undesirable phenotypic traits in organisms, (ii) creation of new organisms by combining two or more beneficial traits of donor organisms, and (iii) synthesis of beneficial products by the new organism for human need.

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Write the advantages and disadvantages of applying such application in the DNA of an organism. a. Drug delivery systems that are based on bacterial or viral host. b. Producing genetically modified organisms to enhance food production. c. Combining two or more genes in one organism to enhance its traits. d. DNA manipulation of certain species to produce organs for harvesting.
When E. coli cells are mixed with recombinant vector DNA and subject to a stress such as heat shock, a small fraction of the cells will take up the plasmid DNA, a process known as :     A. Ligation.   B. Transformation.   C. Transfection.   D. Digestion.
For each situation, write the letter of the technique that would be most helpful; A. DNA editing A doctor wants to know if a patient has an inherited using CRISPR B. DNA replication using PCR C. DNA analysis through genetic testing D. DNA insertion 16. disorder. I A scientist needs many copies of a gene to conduct an 17. experiment. A genetic engineer wants to replace a defective copy of a gene with a functional copy in a chromosome. 18. into bacteria as a plasmid A medical researcher needs many copies of a protein 19. (insulin) to be produced to use in a medical treatment. A researcher crossed two purebred shrubs of the same species. One produces a fruit with a thin skin, and one produces a fruit with a thick skin. All of the plants resulting from the cross produce fruits with thick skins. Enter one letter in each blanks (19 & 20) to correctly complete the sentences. ninate Education TM, Inc.
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Molecular Techniques: Basic Concepts; Author: Dr. A's Clinical Lab Videos;https://www.youtube.com/watch?v=7HFHZy8h6z0;License: Standard Youtube License