Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 3MTF

Summary Introduction

Introduction:

Polymerase chain reaction (PCR) is a rapid method involved in the amplification of a specific segment of the DNA molecule. This technique is used to generate a billion copies of the identical molecule of the DNA segment within a few hours (nearly 30 cycles). It is used for clinical studies and the evaluation of genomic variations. The important components used in PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. Denaturation (separation of double-stranded DNA into two single-strands of DNA), annealing (binding of primers to ends of single-stranded DNA), and extension (addition of dNTPs at 3’ site of the primer by DNA polymerase) are the three main steps of PCR in each cycle. The amplified products are analyzed using electrophoresis technique.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

You are studying the effect of directional selection on body height in three populations (graphs a, b, and c below). (a) What is the selection differential? Show your calculation. (2 pts) (b) Which population has the highest narrow sense heritability for height? Explain your answer. (2 pts) (c) If you examined the offspring in the next generation in each population, which population would have the highest mean height? Why? (2 pts) (a) Midoffspring height (average height of offspring) Short Short Short Short (c) Short (b) Short Tall Short Tall Short Short Tall Midparent height (average height of Mean of population = 65 inches Mean of breading parents = 70 inches Mean of population = 65 inches Mean of breading parents = 70 inches Mean of population = 65 inches Mean of breading parents = 70 inches

P You are studying a population of 100 flowers that has two alleles at a locus for flower color, blue (B) and green (G). There are 15 individuals with the BB genotype, 70 individuals with the BG genotype, and 15 individuals with the GG genotype. (a) What are the allele frequencies of B and G in the starting population? Show your calculations. (2 pts) (b) Is this population in Hardy-Weinberg equilibrium? Show your calculations. (3 pts) 12pt v Paragraph BIU UA AV & VT2V f CO V

In a natural population of outbreeding plants, the variance of the total number of seeds per plant is 16. From the natural population, 20 plants are taken into the laboratory and developed into separate true-breeding lines by self- fertilization-with selection for high, low, or medium number of seeds-for 10 generations. The average variance in the tenth generation in each of the 20 sets is about equal and averages 5.8 across all the sets. Estimate the broad-sense heritability for seed number in this population. (4 pts) 12pt v Paragraph BIUA V V T² v B ① O words