Chapter 8, Problem 5MTF

Summary Introduction

Introduction

The gel electrophoresis method is used to separate the genetic material or PCR amplified products based on their molecular size, shape, and electrical charge. This technique helps to identify and isolate genes of interest or DNA fragments. Such DNA sequences are used to insert into donor organisms through vectors to create genetically modified organisms (GMOs) or serve as a template for PCR amplification or construction of the gene library. The electrophoresis technique is involved to evaluate PCR samples for clinical or genetic diagnosis. Also, it is used for the separation of restricted DNA or RNA samples before transfer in Southern blotting or Northern blotting, respectively.