To answer: The number of DNA molecules synthesized in PCR reaction using 15 DNA helices in 15 cycles. Introduction: PCR technique is a rapid method used to amplify the huge amount of a specific segment of the DNA molecule. It is used to generate a billion copies of identical molecules of the DNA segment within few hours. It is used to analyze genomic variation and mainly involved in clinical studies. The important components used for PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. There are nearly 30 cycles are involved in PCR to produce numerous copies of exact replicas. The main steps of PCR in each cycle: Denaturation: Separation of double-stranded DNA into two single-strand DNA molecules by cleaving the hydrogen bonds at 94°C. Annealing: Binding of primers (20 - 30 nucleotides long) to ends of ssDNA molecules at 65°C. Extension: Addition of dNTPs at 3’ site of the primer by DNA polymerase to synthesize complementary stand of ssDNA at 72°C.

Question

Want to see the full answer?

Answer 1

Question

Chapter 8, Problem 6CT

Summary Introduction

To answer:

The number of DNA molecules synthesized in PCR reaction using 15 DNA helices in 15 cycles.

Introduction:

PCR technique is a rapid method used to amplify the huge amount of a specific segment of the DNA molecule. It is used to generate a billion copies of identical molecules of the DNA segment within few hours. It is used to analyze genomic variation and mainly involved in clinical studies. The important components used for PCR reactions are DNA, primer, deoxyribonucleotide triphosphates (A, T, G, and C) or dNTPs, Taq DNA polymerase, MgCl2, and buffer. There are nearly 30 cycles are involved in PCR to produce numerous copies of exact replicas.

The main steps of PCR in each cycle:

Denaturation: Separation of double-stranded DNA into two single-strand DNA molecules by cleaving the hydrogen bonds at 94°C.

Annealing: Binding of primers (20 - 30 nucleotides long) to ends of ssDNA molecules at 65°C.

Extension: Addition of dNTPs at 3’ site of the primer by DNA polymerase to synthesize complementary stand of ssDNA at 72°C.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

Cellular DNA replication uses the enzymes for the process while PCR uses thermal denaturation that helps seperate DNA strands. I need a picture that shows the difference between them two using the statement above.

The way PCR amplifies DNA is similar to the doubling in apopulation of growing bacteria—a single DNA strand is used tosynthesize 2 DNA strands, which become 4, then 8, then 16, etc. If acomplete cycle takes 3 minutes, how many strands of DNA wouldtheoretically be present after 10 minutes? After 1 hour?

During PCR, the reaction mixture cycles through three temperatures (for example 94, 60, and 72 degrees Celsius) multiple times. What happens during the 94 degree part of a cycle? Group of answer choices Double-stranded DNA denatures and becomes single-stranded. DNA polymerase synthesizes daughter DNA molecules. An oligonucleotide primer anneals to a template DNA strand.