Microbiology: Principles and Explorations
10th Edition
ISBN: 9781119390114
Author: Black
Publisher: WILEY
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Textbook Question
Chapter 8, Problem 4.3SC
Why are nitrates and nitrites, which have been added to sausages to retard bacterial growth and thereby prevent food poisoning, a dnager to humans?
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Chapter 8 Solutions
Microbiology: Principles and Explorations
Ch. 8 - Compare and contrast chromosomes in prokaryotes...Ch. 8 - DNA is not always the gemetic material. What are...Ch. 8 - How could mutations give rise to new alleles of a...Ch. 8 - How does trandlation differ from transcription?Ch. 8 - Distinguish between leading and lagging strands.Ch. 8 - What do 5 and 3 refer to? How do they determine...Ch. 8 - Contrast the three kinds of RNA. Does DNA make all...Ch. 8 - What feeds back in feedback inhibition? What does...Ch. 8 - What is the inducer for the lac operon?Ch. 8 - Compare enzyme induction and enzyme repression.
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- Chlorambucil is a nitrogen mustard. What is the structure of its covalent adduct to DNA?arrow_forwardChemical analysis shows that a nucleic acid sample contains A, U, C, and G. Is this DNA or RNA? Why?arrow_forwardUse the figure of a plasmid below to answer the following questions. 43) Which enzyme was used to produce the molecule in the figure above? A) RNA polymerase C) a restriction enzyme (endonuclease) B) ligase D) DNA polymerasearrow_forward
- In DNA extraction where ethanol was used why does the DNa floatarrow_forwardThere are 6 parts to this question: This is a follow up to the prior question regarding the replication of the DNA strand below. The DNA strand is here for your reference and you do not need to do anything with or to it. TC GATATCGG AGCTATAGCC c) what enzyme separated the parental DNA template strands, d) what bonds were broken? e) what enzyme replicates DNA f) before DNA can be replicated/copied, what must be laid down to allow the enzyme in "e" to replicated the DNA (be specific)? g) our DNA is replicated in many "pieces", what enzyme connects these many "pieces" into one continuous DNA strand that becomes the sister chromatid? h) during what specific phase of the cell cycle does this DNA replication process occur? (This should be a review question from last topics we covered).arrow_forwardIn standard agarose gels used for the analysis of DNA, linear DNA fragments are separated on the basis of their... a) Size b) Charge c) Buoyancy d) Color e) Smellarrow_forward
- PCR can be used __________. a) to increase the number of specific DNA fragments b) to modify the genome c) to obtain the sequence of the DNAarrow_forwardYou are trying to determine the base content for a number of samples in the lab (mouse DNA, bacterial DNA, single-stranded DNA virus and double-stranded RNA virus). Unfortunately, you failed to use an alcohol-proof marker and during the ethanol precipitation of your samples the labels came off the tubes. You finish the experiment anyway and try to use the data to determine the samples. Can you determine the source of each sample based on your results? Briefly explain how you come to your designations. Sample % adenine % cytosine % guanine % thymine % uracil A 18 32 32 18 0 B 26 18 32 24 0 C 31 19 19 0 31 D 10 40 40 10 0arrow_forwardA scientist inserts a radioactively labeled DNA molecule into a bacterium. The bacterium replicates this DNA molecule and distributes one daughter molecule (double helix) to each of two daughter cells. How much radioactivity will the DNA in each of the two daughter cells contain? Why?arrow_forward
- Does each sample contain pure DNA? How do you know?arrow_forwardA DNA strand was sequenced using the Sanger method (https://www.youtube.com/watch?v=KTstRrDTmWI). The reaction tube contained the DNA strand, fluorescently labelled dideoxynucleotide triphosphates (ddATP – yellow, ddGTP – green, ddCTP – blue, ddTTP - red), deoxynucleotide triphosphates, DNA polymerase, or its Klenow fragment. Synthesis of DNA is allowed to proceed, and the results are shown on the right: 15 14 13 12 11 10 (a) What is the sequence of the copy and the template strands? (b) If the template strand were in the 5'-3' direction, what will be the sequence of the DNA copy? Nucleotide Lengtharrow_forwardDefine nucleic acid. What are the best sample sources that can be used in the extraction of nucleic acids? Give examples (2) of nucleic acids. Differentiate RNA from DNA in terms of their chemical properties.arrow_forward
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