Anatomy & Physiology: The Unity of Form and Function
8th Edition
ISBN: 9781259277726
Author: Kenneth S. Saladin Dr.
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 7.3, Problem 10BYGO
Describe the stages of intramembranous ossification. Name a bone that is formed in this way.
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1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Chapter 7 Solutions
Anatomy & Physiology: The Unity of Form and Function
Ch. 7.1 - Name at least five tissues found in a boneCh. 7.1 - Prob. 2BYGOCh. 7.1 - Prob. 3BYGOCh. 7.1 - Prob. 4BYGOCh. 7.1 - The branch of medicine and biology that deals with...Ch. 7.1 - Prob. 2AYLOCh. 7.1 - Prob. 3AYLOCh. 7.1 - Prob. 4AYLOCh. 7.1 - Prob. 5AYLOCh. 7.1 - Other anatomical features of a long bone including...
Ch. 7.1 - Prob. 7AYLOCh. 7.2 - Suppose you had unlabeled electron micrographs of...Ch. 7.2 - Name three organic components of the bone matrix.Ch. 7.2 - What are the mineral crystals of bone called, and...Ch. 7.2 - Sketch a cross section of an osteon and label its...Ch. 7.2 - Prob. 9BYGOCh. 7.2 - Histology of Osseous Tissue 1. The four cell type...Ch. 7.2 - Organic and inorganic components of the bone...Ch. 7.2 - Osteon structure and the relationship of osteonic...Ch. 7.2 - The route by which nerves and blood vessels...Ch. 7.2 - Prob. 5AYLOCh. 7.2 - Prob. 6AYLOCh. 7.3 - Describe the stages of intramembranous...Ch. 7.3 - Describe how a cartilage model is transformed into...Ch. 7.3 - Prob. 12BYGOCh. 7.3 - Prob. 13BYGOCh. 7.3 - Stages of intramembranous ossification; some bones...Ch. 7.3 - Prob. 2AYLOCh. 7.3 - Prob. 3AYLOCh. 7.3 - How stresses on bones remodel item throughout...Ch. 7.4 - Prob. 14BYGOCh. 7.4 - Prob. 15BYGOCh. 7.4 - Prob. 16BYGOCh. 7.4 - Prob. 17BYGOCh. 7.4 - Prob. 1AYLOCh. 7.4 - Prob. 2AYLOCh. 7.4 - Prob. 3AYLOCh. 7.4 - The role of the skeleton as a calcium reservoir in...Ch. 7.4 - Prob. 5AYLOCh. 7.4 - Prob. 6AYLOCh. 7.4 - Prob. 7AYLOCh. 7.4 - Prob. 8AYLOCh. 7.4 - Prob. 9AYLOCh. 7.4 - Prob. 10AYLOCh. 7.5 - Prob. 18BYGOCh. 7.5 - Prob. 19BYGOCh. 7.5 - Prob. 20BYGOCh. 7.5 - Prob. 1AYLOCh. 7.5 - Causes of osteoporosis: its risk factor,...Ch. 7 - Which cells have a ruffled border and secrete...Ch. 7 - Prob. 2TYRCh. 7 - Prob. 3TYRCh. 7 - Osteoclasts are most closely related, by common...Ch. 7 - The walls between cartilage lacunae break down in...Ch. 7 - Which of these is not an effect of PTH? a. rise in...Ch. 7 - Prob. 7TYRCh. 7 - One long bone meets another at its a. diaphysis....Ch. 7 - Calcitriol is made from a. calcitonin. b....Ch. 7 - One sign of osteoporosis is a. osteosarcoma. b....Ch. 7 - Calcium phosphate crystallizes in bone as a...Ch. 7 - Prob. 12TYRCh. 7 - Prob. 13TYRCh. 7 - Prob. 14TYRCh. 7 - Prob. 15TYRCh. 7 - Prob. 16TYRCh. 7 - Prob. 17TYRCh. 7 - Prob. 18TYRCh. 7 - Prob. 19TYRCh. 7 - Prob. 20TYRCh. 7 - Prob. 1BYMVCh. 7 - Prob. 2BYMVCh. 7 - Prob. 3BYMVCh. 7 - Prob. 4BYMVCh. 7 - ortho-Ch. 7 - Prob. 6BYMVCh. 7 - Prob. 7BYMVCh. 7 - Prob. 8BYMVCh. 7 - spic-Ch. 7 - topo-Ch. 7 - Prob. 1WWTSCh. 7 - Prob. 2WWTSCh. 7 - Prob. 3WWTSCh. 7 - The growth zone of the lone bones of adolescents...Ch. 7 - Prob. 5WWTSCh. 7 - Prob. 6WWTSCh. 7 - Whats Wrong with These Statements? 7. The protein...Ch. 7 - Prob. 8WWTSCh. 7 - Prob. 9WWTSCh. 7 - Prob. 10WWTSCh. 7 - Most osteocytes of an osteon are far removed from...Ch. 7 - Prob. 2TYCCh. 7 - How does the regulation of blood calcium...Ch. 7 - Describe how the arrangement of trabeculae in...Ch. 7 - Identify two bone diseases you would expect to see...
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