EBK CAMPBELL BIOLOGY
10th Edition
ISBN: 9780136539414
Author: Reece
Publisher: VST
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Textbook Question
Chapter 7, Problem 7TYU
EVOLUTION CONNECTION Paramecium and other unicellular eukaryotes that live in hypotonic environments have cell membranes that limit water uptake, while those living in isotonic environments have membranes that are more permeable to water. Describe what water regulation adaptations might have evolved in unicellular eukaryotes in hypertonic habitats such as the Great Salt Lake and in habitats with changing salt concentration.
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a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
1) Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
2) You used aseptic technique during this lab. Why is it important to work in a sterile manner when working with bacteria in the lab?
3) Why are the cells incubated at 42°C?
Chapter 7 Solutions
EBK CAMPBELL BIOLOGY
Ch. 7.1 - VISUAL SKILLS Carbohydrates are attached to...Ch. 7.1 - WHAT IF? How would the membrane lipid composition...Ch. 7.2 - What property allows O2 and CO2 to cross a lipid...Ch. 7.2 - VISUAL SKILLS Examine Figure 7.2. Why is a...Ch. 7.2 - MAKE CONNECTIONS Aquaporins exclude passage of...Ch. 7.3 - How do you think a cell performing cellular...Ch. 7.3 - WHAT IF? If a Paramecium swims from a hypotonic...Ch. 7.4 - Sodium-potassium pumps help nerve cells establish...Ch. 7.4 - VISUAL SKILLS Compare the sodium-potassium pump...Ch. 7.4 - MAKE CONNECTIONS Review the characteristics of...
Ch. 7.5 - As a cell grows, its plasma membrane expands. Does...Ch. 7.5 - DRAW IT Return to Figure 7.9, and circle a patch...Ch. 7.5 - Prob. 3CCCh. 7 - In what ways are membranes crucial to life?Ch. 7 - How do aquaporins affect the permeability of a...Ch. 7 - What happens to a cell placed in a hypertonic...Ch. 7 - ATP is not directly involved in the functioning of...Ch. 7 - Which type of endocytosis involves the binding of...Ch. 7 - In what way do the membranes of a eukaryotic Cell...Ch. 7 - According to the fluid mosaic model of membrane...Ch. 7 - Which of the following factors would tend to...Ch. 7 - Which of the following processes includes all the...Ch. 7 - Prob. 5TYUCh. 7 - DRAW IT An artificial "cell" consisting of an...Ch. 7 - EVOLUTION CONNECTION Paramecium and other...Ch. 7 - Prob. 8TYUCh. 7 - SCIENCE, TECHNOLOGY, AND SOCIETY Extensive...Ch. 7 - WRITE ABOUT A THEME: INTERACTIONS A human...Ch. 7 - SYNTHESIZE YOUR KNOWLEDGE In the supermarket,...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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