Microbiology with Diseases by Body System (4th Edition)
4th Edition
ISBN: 9780321918550
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 7, Problem 1VI
On the figure below, label DNA polymerase I, DNA polymerase III, helicase, lagging strand, leading strand, ligase,
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The figure below depicts various elements of the eukaryotic replication machinery in action. Enter the name for the protein depicted
by each box.
Box A
Box B
Box C
Box D
Box E
Box F
DNA polymerase on
lagging strand (just
finishing an Okazaki
fragment)
F
Maintains polymerase association with DNA
Enzyme extends separation of DNA strands
Synthesizes RNA fragments that hybridize to DNA
Relaxes supercoiled DNA ahead of replication fork
Maintains DNA is single stranded state
Promotes binding of processivity factors to DNA
Newly synthesized strand
pocoar
Leading-strand template
A
New Okazaki fragment
RNA
primer
E
Lagging-strand
template
DNA polymerase
on leading strand
B
C
D
Saaragon
- Next Okazaki fragment
will start here
Parental DNA helix
Describe how replication makes copies of DNA.
Include the following:
helicase
polymerase
primase and primer
ligase
leading strand
lagging strand
base pairing rule (a-t, c-g)
nucelotide
nucleus
semiconservative
Assign the labels to their appropriate locations in the flowchart below, indicating the sequence of DNA replication events in the production of fragment B. (Note that pol I stands for DNA polymerase I, and pol III stands for DNA polymerase III.)
Chapter 7 Solutions
Microbiology with Diseases by Body System (4th Edition)
Ch. 7 - DNA replication requires a large amount of energy,...Ch. 7 - Vibrio vulnificus Infection Greg enjoyed Floridas...Ch. 7 - Prob. 2TMWCh. 7 - Prob. 3TMWCh. 7 - Why is the genetic ancestry of microbes much more...Ch. 7 - Prob. 1CCSCh. 7 - Which of the following is most likely the number...Ch. 7 - Which of the following is a true statement...Ch. 7 - A plasmid is ___________. a. a molecule of RNA...Ch. 7 - Prob. 4MC
Ch. 7 - Prob. 5MCCh. 7 - Which of the following molecules functions as a...Ch. 7 - Prob. 7MCCh. 7 - Prob. 8MCCh. 7 - The Ames test ___________. a. uses auxotrophs and...Ch. 7 - Which of the following methods of DNA repair...Ch. 7 - Prob. 11MCCh. 7 - Prob. 12MCCh. 7 - Which of the following statements is true? a....Ch. 7 - Prob. 14MCCh. 7 - Although two cells are totally unrelated, one cell...Ch. 7 - Prob. 16MCCh. 7 - Prob. 17MCCh. 7 - Prob. 18MCCh. 7 - Prob. 19MCCh. 7 - Prob. 20MCCh. 7 - Prob. 21MCCh. 7 - Prob. 22MCCh. 7 - Prob. 23MCCh. 7 - Before mutations can affect a population...Ch. 7 - Prob. 25MCCh. 7 - Fill in the Blanks 1. The three steps in RNA...Ch. 7 - Fill in the Blanks 2. A triplet of mRNA...Ch. 7 - Fill in the Blanks 3. Three effects of point...Ch. 7 - Fill in the Blanks 4. Insertions and deletions in...Ch. 7 - Fill in the Blanks 5. An operon consists of...Ch. 7 - Prob. 6FIBCh. 7 - Prob. 7FIBCh. 7 - Fill in the Blanks 8. A gene for antibiotic...Ch. 7 - Fill in the Blanks 9. ______ are nucleotide...Ch. 7 - Fill in the Blanks 10. ____________ is a...Ch. 7 - Fill in the Blanks 11.________ RNA carries amino...Ch. 7 - Fill in the Blanks 12. ______ RNA and ______ RNA...Ch. 7 - How does the genotype of a bacterium determine its...Ch. 7 - List several ways in which eukaryotic messenger...Ch. 7 - Compare and contrast intrans and exons.Ch. 7 - Polypeptide synthesis requires large amounts of...Ch. 7 - Describe the operon model of gene regulation.Ch. 7 - Prob. 6SACh. 7 - Prob. 7SACh. 7 - Describe the formation and function of mRNA, rRNA,...Ch. 7 - Prob. 9SACh. 7 - Explain the central dogma of genetics.Ch. 7 - Compare and contrast the processes of...Ch. 7 - Fill in the following table:Ch. 7 - On the figure below, label DNA polymerase I, DNA...Ch. 7 - Prob. 2VICh. 7 - The drugs ddC and AZT are used to treat AIDS....Ch. 7 - Prob. 1CTCh. 7 - A scientist uses a molecule of DNA composed of...Ch. 7 - Explain why an insertion of three nucleotides is...Ch. 7 - How could scientists use siRNA to turn off a...Ch. 7 - Prob. 5CTCh. 7 - Prob. 6CTCh. 7 - Prob. 7CTCh. 7 - Prob. 8CTCh. 7 - Corynebacterium diphtheriae, the causative agent...Ch. 7 - Prob. 10CTCh. 7 - The endosymbiotic theory states that mitochondria...Ch. 7 - Hydrogen bonds between complementary nucleotides...Ch. 7 - On average, RNA polymerase makes one error for...Ch. 7 - We have seen that wobble makes the genetic code...Ch. 7 - If a scientist synthesizes a DNA molecule with the...Ch. 7 - What DNA nucleotide triplet codes for codon UGU?...Ch. 7 - Suppose you want to insert into your dog a gene...Ch. 7 - Using the following terms, fill in the following...
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- The image shows a replication fork with template DNA strands, new DNA strands, and some replication proteins. Label the mage by moving the terms or descriptive phrases to the appropriate targets. lagging strand (or Okazaki fragment) Answer Bank protein that synthesizes RNA primer DnaB helicase DNA gyrase (topoisomerase)arrow_forwardWhat proteins are crucial for creating and maintaining DNA replication forks? Choose the best explanation. Question 2 options: Helicase creates the replication fork; primase keeps the single strands from closing shut. Helicase creates the replication fork; single-strand binding proteins keep the single strands from reuniting. Ligase creates the replication fork; DNA polymerase II keeps the single strands from reuniting. Helicase creates the replication fork; ligase keeps the single strands from closing shut.arrow_forwardDefine the following terms related to DNA replication: origin of replication, helicase, single-strand binding proteins, topoisomerase, primase, RNA primer, DNA polymerase III, DNA polymerase I, and DNA ligase.arrow_forward
- Fill in the blank spaces below with the most appropriate terms. The word bank is not provided. DNA replication in bacterium Escherichia coli begins at a site in the DNA called At the replication fork, the strand is synthesized continuously while the strand is synthesized discontinuously (in fragments). The new DNA strand, which is synthesized discontinuously, initially consists of short DNA pieces that are called A short RNA primer at the beginning of each of the DNA fragments is synthesized by an enzyme called and this RNA primer is later removed by the enzyme called using its activity. Single-strand breaks (nicks) that are left behind in this process are sealed by the enzyme called A Moving to another question w!l save this resporse Quebon 4 Inarrow_forwardDescribe the role of the proteins involved with DNA replication. Also identify if the protein is involved the leading strand, lagging strand or both. DNA Ligase DNA Polymerase I DNA Polymerase III Helicase Primase Single-stranded DNA-binding proteins Topoisomerasearrow_forwardIn the following diagram of a replication fork, primers are shown as thick black lines and newly synthesized DNA is shown as squiggly lines. Enzymes are shown as circles or boxes. Click on DNA polymerase I 3' 5' 7 c 34 % G Search or type URL 3' MacBook Pro & 5' + (: * II in 3 5' 3' +arrow_forward
- Name the bacterial enzyme that takes over new strand synthesis in replication after the primer is formed. Please include any number that is applicable. DNA polymerase omega DNA polymerase II DNA Polymerase epsilon DNA polymerase delta O DNA Polymerase Iarrow_forwardMatch each term with the correct letterarrow_forwardIdentify the various types of DNA repair mechanisms known to counteract the effects of UV radiation. Drag the terms on the left to the appropriate blanks on the right to complete the sentences. Reset Help SoS repair is dependent on a photon-activated enzyme that cleaves thymine dimers. excision repair is the process by which an endonuclease clips out UV-induced dimers, DNA photoreactivation repair polymerase III fills in the gap, and DNA ligase rejoins the phosphodiester backbone. recombinational repair uses the corresponding region on the umdamaged parental strand of the same polarity. is a process in E. coli that induces error-prone DNA replication in an effort to fill gaps by inserting random nucleotides.arrow_forward
- Match each DNA Replication enzymes on the left with its function I) DNA Ligase II) DNA Polymerase II III) DNA Polymerase I IV) DNA Helicase V) Primase VI) DNA Polymerase III Proof reads DNA after replication and checks for mistakes Breaks hydrogen bonds holding adjacent base pairs together Synthesizes complimentary strands of DNA during DNA replication Builds RNA primers Removes RNA primers and replaces them with DNA nucleotides during replication Joins adjacent DNA nucleotides to each otherarrow_forwardMatch the following descriptions with the enzymes involved in DNA replication. 1. Adds an RNA primer to begin elongation 2. Removes the RNA primer from the beginning of the newly constructed strands 3. Splices lagging strand segments 4. Cleaves the rung of the DNA double helix ladder Description: DNA DNA Helicase Primase Enzyme: Polymerase Ligasearrow_forwardThe proteins and enzymes listed below are all required for DNA replication in E. coli, but they are listed in a random order. Determine the correct order in which they function in replication, by selecting the correct number from the drop-down menu in each case, with 1 being first and 6 being last.arrow_forward
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