Microbiology: Principles and Explorations
9th Edition
ISBN: 9781118743164
Author: Jacquelyn G. Black, Laura J. Black
Publisher: WILEY
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Textbook Question
Chapter 7, Problem 10SQ
For the lac operon, match the following:
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Determine the outcome for the lac operon genotype shown below:
I*p*o°z*y/I*p*o*z*x*
Assume lactose is absent.
Select one:
O Functional beta-galactosidase and functional permease are produced.
O Only functional beta-galactosidase is produced. Functional permease is NOT produced.
O Only functional permease is produced. Functional beta-galactosidase is NOT produced.
O Functional beta-galactosidase is NOT produced. Functional permease is NOT produced.
In terms of the trp operon, differentiate between two normal bacterial cultures, one grown in a medium supplied with tryptophan and the other medium without tryptophan.
The lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a
single polycistronic mRNA. The new lac strain of Escherichia coli are as shown below:
P+O+Z+Y+A+// P+O+Z+Y+A¯
(i)
Illustrate how lactose induces the gene expression in lac 1 $// I-.
(ii)
Explain the function of the lacP and laco in the bacterial operon.
Chapter 7 Solutions
Microbiology: Principles and Explorations
Ch. 7 - Compare and contrast chromosomes in prokaryotes...Ch. 7 - DNA is not always the gemetic material. What are...Ch. 7 - How could mutations give rise to new alleles of a...Ch. 7 - How does trandlation differ from transcription?Ch. 7 - Distinguish between leading and lagging strands.Ch. 7 - What do 5 and 3 refer to? How do they determine...Ch. 7 - Contrast the three kinds of RNA. Does DNA make all...Ch. 7 - What feeds back in feedback inhibition? What does...Ch. 7 - What is the inducer for the lac operon?Ch. 7 - Compare enzyme induction and enzyme repression.
Ch. 7 - If you had be exposed to a mutagen,would you...Ch. 7 - How can a change in geneotype fail to produce a...Ch. 7 - Why are nitrates and nitrites, which have been...Ch. 7 - Cathy develops a painful cluster of blister-like...Ch. 7 - Prokaryotes usually have just one chromosome and...Ch. 7 - During the early stages of development of the...Ch. 7 - Suppose you are a medical technician working in a...Ch. 7 - Match the following terms with their descriptions:Ch. 7 - Prob. 2SQCh. 7 - Prob. 3SQCh. 7 - Match the following terms with their respective...Ch. 7 - From the DNA template sequence 3-ATGCAGTAG-5, what...Ch. 7 - What type of RNA carries and transfers amino acids...Ch. 7 - What type of RNA carries the genetic information...Ch. 7 - Which of the following statements does not...Ch. 7 - Prob. 9SQCh. 7 - For the lac operon, match the following:Ch. 7 - Prob. 11SQCh. 7 - A frameshift mutation occurs following the: (a)...Ch. 7 - Prob. 13SQCh. 7 - Which of the following schemes for molecular...Ch. 7 - Suppose a point mutation occurred in the third...Ch. 7 - Prob. 16SQCh. 7 - (a) Why are bacteria useful in the study of...Ch. 7 - DNA damage in the form of dimers induced by UV...Ch. 7 - Prob. 19SQCh. 7 - Name and describe the effects of the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A strain of E. coli has the genotypes shown below at the lac operon, where I = regulator gene, P = promoter, O = operator, Z = βgalactosidase gene, and Y = permease gene. The superscript+ indicates a wild-type allele,c indicates a constitutive mutation, and− indicates a defective mutation. For each genotype, indicate whether the enzyme will be synthesized or not synthesized when lactose is present or absent by placing a + for synthesis occurring and a − for synthesis not occurring in the appropriate blank. Explain your reasoning for each answer.arrow_forwardA number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the highest beta-galactosidase enzyme activity, when grown in the lactose medium. O CAP+ r* p* o* z O CAP* I P* o* z* O CAP* r* P O* z* O CAP I P* O z*arrow_forwardFor each of the following conditions in the Lac Operon, state whether transcription will occur or not and state why. Include all the major actors including glucose, lactose, cAMP and CAP(CPR). Key + (present) - (absent) 1) Glucose-; Lactose- 2) Glucose+; Lactose- 3) Glucose+; Lactose+ 4) Glucose-; Lactose+arrow_forward
- Explain how the lac operon can be used to express a recombinant protein in an E. coli cell. Explain why the B-galactosidase gene is made in two pieces with the a and 2 parts of the enzyme.arrow_forwarda) State the functions of the subunits of RNA polymerase and describe transcription initiation. b)Draw a diagram to illustrate the lac operon and explain how it functions in the presence of, i) glucose and ii) lactose in the culture mediumarrow_forwardThe lac operon consists of three structural genes, lacZ, lacY and lacA that are transcribed as a single polycistronic mRNA. You are given a new strain of Escherichia coli with the following lac operon genotype: p+0°Z•Y*A +// P*O*Z*Y+ A- (i) Explain how the lac I gene affects gene expression. (ii) Explain the function of the lacP in the bacterial operon. (iii) Which part of the lac operon is cis-dominant? Explain.arrow_forward
- The streptolysin S toxin made by S. pyogenes is encoded by a 9-gene operon, sagABCDEFGHI. Thinking about what a 3-line diagram would look like for this operon, answer the following questions. Write numeric answers only. For example, if your answer is 6 promoters, write only 6. 1) How many promoters control the expression of these genes? 2) How many locations does RNA Polymerase bind to get full expression of these genes? 3) How many ribosome binding sites are needed for full protein expression? 4) How many start codons will be needed for full protein expression? 5) How many mRNA strands will be produced with full operon expression? 6) How many proteins will be produced with full protein expression? 1arrow_forwardExplain why large amounts of transcript result when a bacterium with a lac Operon is in a medium with no glucose and an abundance of lactose.arrow_forwardWhich of the following lac operon genotypes would allow for functional versions of all the structural enzymes of the lac operon to be expressed constitutively even in the absence of lactose? Group of answer choices I+ O+ Z+ Y+ A+ I- O+ Z- Y- A- I+ OC Z+ Y+ A+ IS O+ Z+ Y+ A+ I+ O+ Z- Y+ A+arrow_forward
- Predict the level of genetic activity of the lac operon as well as the status of the lac repressor and the CAP protein under the cellular conditions listed in the accompanying table. Lactose Glucose (a) - - (b) + - (c) - + (d) + +arrow_forwardSupply the words “off" or “on" in the blanks below: In an inducible operon, transcription is normally and must be turned In a repressible operon, transcription is normally and must be turned Briefly describe the overall function of the structural genes in the lac operon. Briefly describe the overall function of the structural genes in the trp operon. For the following descriptions of the bacterial lac operon, determine if lactose is present (P) or absent (A). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Allolactose is bound to the repressor For the following descriptions of the bacterial trp operon, determine if tryptophan levels are high (H) or low (L). The repressor is bound to the operator. The repressor is inactive. RNA polymerase transcribes the structural genes. Tryptophan is bound to the repressor.arrow_forwardA number of mutations affect the expression of the lac operon in E. coli. The genotypes of several E. coli strains are shown below. ("+" indicates a wild-type gene with normal function and "-" indicates a loss-of-function allele.) Please predict which of the following strains would have the lowest beta-galactosidase enzyme activity, when grown in the lactose medium. Orpt o* z* r* Orpt ot z* Y OrptoztY Orrotzr OrPotz*Yarrow_forward
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