Human Anatomy
6th Edition
ISBN: 9781260399820
Author: SALADIN, Kenneth
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 6, Problem 6.3.4AYLO
Structural differences between the endochondral bones of adults and children, especially with regard to the epiphysial plates
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 6 Solutions
Human Anatomy
Ch. 6.1 - Prob. 1BYGOCh. 6.1 - List three or more functions of the skeletal...Ch. 6.1 - Prob. 3BYGOCh. 6.1 - Prob. 4BYGOCh. 6.1 - Prob. 5BYGOCh. 6.2 - Considering the function of osteoblasts, what...Ch. 6.2 - Suppose you had unlabeled electron micrographs of...Ch. 6.2 - Prob. 7BYGOCh. 6.2 - Prob. 8BYGOCh. 6.2 - Sketch a cross section of an osteon and label its...
Ch. 6.2 - What are the two kinds of bone marrow? What does...Ch. 6.3 - Prob. 1AWYKCh. 6.3 - Describe the stages of endochondral ossification....Ch. 6.3 - Prob. 12BYGOCh. 6.3 - How does Wolff’s law explain some of the...Ch. 6.3 - Prob. 14BYGOCh. 6.3 - Prob. 15BYGOCh. 6.4 - Name and describe any five types of bone...Ch. 6.4 - What is a callus? How does it contribute to...Ch. 6.4 - Prob. 18BYGOCh. 6 - Components of the skeletal system, including but...Ch. 6 - Seven function of the skeletal systemCh. 6 - The constituents of a bone, including but not...Ch. 6 - Prob. 6.1.4AYLOCh. 6 - The spatial relationship between compact and...Ch. 6 - Prob. 6.1.6AYLOCh. 6 - Prob. 6.1.7AYLOCh. 6 - Prob. 6.2.1AYLOCh. 6 - The spectalized structure of an osteocyte and how...Ch. 6 - Prob. 6.2.3AYLOCh. 6 - The composition of the bone matrix and the...Ch. 6 - Prob. 6.2.5AYLOCh. 6 - Prob. 6.2.6AYLOCh. 6 - The two kinds of bone marrow, their locations in...Ch. 6 - The two modes of bone development (ossification)...Ch. 6 - Prob. 6.3.2AYLOCh. 6 - The stages of endochondral ossification, replacing...Ch. 6 - Structural differences between the endochondral...Ch. 6 - The zones of tissue found in the metaphysis of a...Ch. 6 - Prob. 6.3.6AYLOCh. 6 - How bones grow in thickness and change shape even...Ch. 6 - Prob. 6.3.8AYLOCh. 6 - The nutrients needed for bone growth and...Ch. 6 - Hormones that regulate bone growth and remodelingCh. 6 - Prob. 6.3.11AYLOCh. 6 - Prob. 6.4.1AYLOCh. 6 - Prob. 6.4.2AYLOCh. 6 - Prob. 6.4.3AYLOCh. 6 - Prob. 6.4.4AYLOCh. 6 - Osteoporosis risk factors, prevention, diagnosis,...Ch. 6 - Causes, signs, and symptoms of rickets and...Ch. 6 - Which cells have a ruffled border and secrete...Ch. 6 - The medullary cavity of a child’s bone may contain...Ch. 6 - The long bones of the limbs grow in length by cell...Ch. 6 - Prob. 4TYRCh. 6 - Prob. 5TYRCh. 6 - Prob. 6TYRCh. 6 - Prob. 7TYRCh. 6 - Prob. 8TYRCh. 6 - Prob. 9TYRCh. 6 - Prob. 10TYRCh. 6 - Prob. 11TYRCh. 6 - Prob. 12TYRCh. 6 - Prob. 13TYRCh. 6 - Prob. 14TYRCh. 6 - Prob. 15TYRCh. 6 - Prob. 16TYRCh. 6 - Prob. 17TYRCh. 6 - Prob. 18TYRCh. 6 - Prob. 19TYRCh. 6 - Prob. 20TYRCh. 6 - Prob. 1BYMVCh. 6 - Prob. 2BYMVCh. 6 - Prob. 3BYMVCh. 6 - Prob. 4BYMVCh. 6 - Prob. 5BYMVCh. 6 - Prob. 6BYMVCh. 6 - Prob. 7BYMVCh. 6 - Prob. 8BYMVCh. 6 - Prob. 9BYMVCh. 6 - State a meaning of each word element and give a...Ch. 6 - Prob. 1WWWTSCh. 6 - Prob. 2WWWTSCh. 6 - Briefly explain why each of the following...Ch. 6 - Prob. 4WWWTSCh. 6 - Briefly explain why each of the following...Ch. 6 - Prob. 6WWWTSCh. 6 - Prob. 7WWWTSCh. 6 - Prob. 8WWWTSCh. 6 - Prob. 9WWWTSCh. 6 - Briefly explain why each of the following...Ch. 6 - Prob. 1TYCCh. 6 - Prob. 2TYCCh. 6 - One of the more common fractures in children and...Ch. 6 - Prob. 4TYCCh. 6 - Prob. 5TYC
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