Prescott's Microbiology
10th Edition
ISBN: 9781259281594
Author: Joanne Willey, Linda Sherwood Adjunt Professor Lecturer, Christopher J. Woolverton Professor
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 5.4, Problem 2RIA
Retrieve, Infer, Apply
What is a proteasome? Why is it important to the proper functioning of the ER?
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Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a target protein that is rich in Cys and Tyr residues. Which of the following techniques should be considered in accurately quantifying the isolated protein?I. Running the isolated protein in a dialysis or GFC set up.II. Using Biuret or BCA assay as the colorimetric quantitation method.III. Using Bradford or Lowry assay as the colorimetric quantitation method.A. I onlyB. II onlyC. I and IIID. I, II and III. Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be morea. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups.b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups.c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups.d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.
Explain the basis for identification using biochemical testing. – Again, discuss enzymatic pathways, how do we make enzymes in a cell, what is the blueprint for an enzyme?
Outline the proofreading processes in amino acidactivation.
Chapter 5 Solutions
Prescott's Microbiology
Ch. 5.1 - MICRO INQUIRY In addition to separating each...Ch. 5.1 - Prob. 1RIACh. 5.1 - Retrieve, Infer, Apply 2. Why is the...Ch. 5.3 - MICRO INQUIRY Which cytoskeletal filament is made...Ch. 5.3 - Prob. 1RIACh. 5.3 - Prob. 2RIACh. 5.3 - Prob. 3RIACh. 5.4 - MICRO INQUIRY Why must proteins be unfolded when...Ch. 5.4 - Prob. 1RIACh. 5.4 - Retrieve, Infer, Apply What is a proteasome? Why...
Ch. 5.4 - Prob. 3RIACh. 5.4 - Prob. 4RIACh. 5.4 - Describe the secretory pathway. To what...Ch. 5.4 - Prob. 6RIACh. 5.5 - Prob. 1MICh. 5.5 - MICRO INQUIRY Which subunit attaches to the rough...Ch. 5.5 - Prob. 1RIACh. 5.5 - Retrieve, Infer, Apply Suggest an explanation for...Ch. 5.5 - Prob. 3RIACh. 5.5 - Prob. 4RIACh. 5.6 - Prob. 1MICh. 5.6 - Describe the structure of aerobic mitochondria,...Ch. 5.6 - Prob. 2RIACh. 5.6 - Prob. 3RIACh. 5.7 - Prepare and label a diagram showing the detailed...Ch. 5.7 - Prob. 2RIACh. 5.7 - How do the structure and mechanism of action of...Ch. 5.8 - Retrieve, Infer, Apply Outline the major...Ch. 5.8 - Retrieve, Infer, Apply What characteristics make...Ch. 5 - Discuss the statement: The most obvious difference...Ch. 5 - Bacterial and archaeal cell size is limited by the...Ch. 5 - Prob. 3CHICh. 5 - Compare the mechanisms by which most eukaryotic...Ch. 5 - Prob. 5CHI
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- Please answer ASAP: What is Shine-Dalgarno sequence? In which groups of microorganisms it is found?arrow_forwardIN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR ABSORBANCE. IS IT POSSIBLE?arrow_forwardpresent briefly the principle of size exclusion chromatography. give a brief description of the use of protein assays.arrow_forward
- Under most in vitro assay conditions, the enzyme is used in catalytic amounts (10 to 10" M). Estimate the concentration of an enzyme in a living cell. Assume that (a) fresh tissue is 80% water and all of it is intracellular, (b) the total soluble protein in a cell represents 15% of the wet weight, (c) all the soluble proteins are enzymes, (d) the average molecular weight of a protein is 150,000, and (e) about 1000 different enzymes are present.arrow_forwardBesides agarosc gel electrophoresis, there is also SDS-PAGE for separation of macromolccules. Differentiate both these methods.arrow_forwardGive typing answer with explanation and conclusion to all partsarrow_forward
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