Biochemistry: Concepts and Connections
1st Edition
ISBN: 9780321839923
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 5, Problem 8P
Given the following peptide
SEPIMAPVEYPK
a. Estimate the net charge at pH 7 and at pH 12. Assume the pKa values given in Table 5.1
b. How many peptides would result if this peptide were treated with (1) cyanogen bromide, (2) trypsin, or (3) chymotrypsin?
c. Suggest a method for separating the peptides produced by chymotrypsin treatment.
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Biochemistry: Concepts and Connections
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