Biochemistry: Concepts and Connections
1st Edition
ISBN: 9780321839923
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 5, Problem 9P
A mutant form of polypeptide hormone angiotensin Il has the amino acid composition (Asp, Arg, Ile, Met, Phe, Pro, Tyr, Val)
The following observations are made.
• Trypsin yields a dipeptide containing Asp and Arg and a hexapeptide with all the rest.
• Cyanogen bromide cleavage yields a dipeptide containing Phe and Pro and a hexapeptide containing all others.
• Chymotrypsin cleaves the hormone into two tetrapeptides, of composition (Asp, Arg, Tyr, Val) and (Ile, Met, Phe, Pro)
• The dipeptide of composition (Pro, Phe) cannot be cleaved by either chymotrypsin or carboxypeptidase
What is the sequence of angiotensin II?
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Biochemistry: Concepts and Connections
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