WHAT IS LIFE? A GUIDE TO BIO 3E+LAUNCHPA
3rd Edition
ISBN: 9781319103316
Author: PHELAN
Publisher: Macmillan Higher Education
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Chapter 5, Problem 13MC
Summary Introduction
Introduction:
Gene therapy is a treatment strategy that is being explored for the treatment of genetic diseases in which an essential gene is lacking in an individual, and a functioning gene is introduced in the patient’s body to make up for it.
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Arrange the following steps involved in Gene cloning in the correct orderi) Isolation of desired geneii) Insertion of isolated gene to the vectoriii) Introduction of recombinant vector to the hostiv)Expression of recombinant gene in hostv) Extraction of recombinant gene producta) i, ii, iii, iv, vb) v, iv, iii, ii, ic) i, iii, ii, iv, vd) iv, v, ii, i, iii
Which of the statements is TRUE about conjugation?
a)
Plasmid DNA is transferred from a donor (F+) cell
b)
It may only be performed by competent cells
c)
DNA is always integrated into recipient chromosome
d)
It may be specialized or generalized
Which of the following is not associated with gene therapy SCID patients
A) utilizes an enzyme to repair the patient’s nonfunctioning ADA gene
B) isolation of patient cells
C) cloning of functioning a D a gene in bacteria
D) use of vector
Chapter 5 Solutions
WHAT IS LIFE? A GUIDE TO BIO 3E+LAUNCHPA
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Ch. 5 - Prob. 11SACh. 5 - Prob. 12SACh. 5 - Prob. 13SACh. 5 - Prob. 14SACh. 5 - Prob. 15SACh. 5 - Prob. 1MCCh. 5 - Prob. 2MCCh. 5 - Prob. 3MCCh. 5 - Prob. 4MCCh. 5 - Prob. 5MCCh. 5 - Prob. 6MCCh. 5 - Prob. 7MCCh. 5 - Prob. 8MCCh. 5 - Prob. 9MCCh. 5 - Prob. 10MCCh. 5 - Prob. 11MCCh. 5 - Prob. 12MCCh. 5 - Prob. 13MCCh. 5 - Prob. 14MCCh. 5 - Prob. 15MCCh. 5 - Prob. 16MCCh. 5 - Prob. 17MC
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- Common vectors used to transfer a piece of DNA into a cloning host are ________. A) artificial chromosomes B) bacteriophages C) All of the choices are correct D) viruses E) plasmidsarrow_forwardWhat's the best definition of gene therapy? a) The introduction of a non-mutated and functional gene into a patient's DNA. b) The delivery of a non-mutated and functional protein into a patient. c) The introduction of a non-mutated and functional gene into a patient's RNA. d) The delivery of drugs that encourage overexpression of a protein to compensate for a mutated protein.arrow_forwardWhich of the following statements is true about a vector?a) Plasmids, phages can be used as vectorsb) All vectors are plasmidsc) Cyanobacteria can also be used as vectorsd) Fungi can also be used as vectorsarrow_forward
- Why must the top agar be supplemented with MgSo4 A) it is required to induce the production of cro repressors B) it is required to induce the production of cI repressors C) it helps stabilize the phage for infection D) it induces the expression of the lamB gene in E. Coliarrow_forwardUsually, bacteria only make tryptophan when tryptophan is absent or available in low concentration. However, a particular bacterial mutation makes tryptophan all the time whether or not tryptophan is present. What could explain this phenotype? A) the terminator hairpin is unable to form B) the antiterminator hairpin is unable to form C) trpE is mutated D) trpD is mutated E) trpA is mutatedarrow_forwardAll of the following apply to Luria and Delbruck’s 1943 study of mutation rates in E. Coli and T1 phage except: A) it served as an example of an inflexible test B) it involved looking for T1-resistant bacteria as an end measure C) it showed that numbers of mutant organisms observed after T 1 phage were added to the culture tended to vary from experiment to experiment D) it rejected the possibility of adaptive mutations E) it supported the concept of spontaneous mutation ratesarrow_forward
- Gene therapy is a technique preferred to cure inherited diseases bya) Repairing the faulty geneb) Introducing the correct copy of the genec) Adding new cells to the bodyd) PCRarrow_forwardOne of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?arrow_forward20 16. Which of the following methods may introduce foreign DNA into a recipient? A) transduction B) conjugation C) transformation D) All of the above 17. F+ strains of Escherichia coli A) have an integrated F factor. B) have the F factor as a plasmid. C) do not have an F factor. D) transfer the F factor to recipient cells at a high frequency. 18. What is the function of the conjugation pilus? A) It contains an F plasmid. B) It carries the chromosome of F- cells. C) It converts F- cells into F+ cells. D) It pulls the F+ and F- cells together. 19. When a transposon is cut from one location in genome and pasted into a new location, it is called replicative transposition A) True B) False (0 20. Insertion sequences are bounded by inverted repeats and encode for transposase. A) True B) False 21. The CRISPR system UM (8 A) recognizes foreign DNA sequences that have previously entered the cell and directs the Cas proteins to destroy them. B) synthesizes gene transfer agents during stationary…arrow_forward
- Generally a plasmid vector contains how many elements?a) 1b) 2c) 3d) 4arrow_forwardIf the mutation has a negligible effect on the function of a gene, it is known as aa) Silent mutationb) Frame shift mutationc) Substitution mutationd) Insertion mutationarrow_forwardWhich is not needed to clone an animal? A)sperm for a donor animal B)nucleus from an adult animal C)host female to develop the embryo D) all the abovearrow_forward
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