WHAT IS LIFE? A GUIDE TO BIO 3E+LAUNCHPA
3rd Edition
ISBN: 9781319103316
Author: PHELAN
Publisher: Macmillan Higher Education
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Chapter 5, Problem 12MC
Summary Introduction
Introduction:
PCR is a technique used in amplifying a DNA (or RNA) sample. In any test involving
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The enzyme that proofreads the new strand of DNA, looking for and correcting mistakes as it replicates is called:
A) helicase
B) DNA polymerase III
C) ligase
D) RNA polymerase
E) primase
The polymerase chain reaction uses Taq polymerase rather than a DNA polymerase from E. coli, because Taq polymerasea) introduces fewer errors during DNA synthesis.b) is heat-stable.c) can initiate DNA synthesis at a wider variety of sequences.d) can denature a double-stranded DNA template.e) is easier to obtain.
Choose the statements below which are correct. There may be more than one correct statement.
A) primers are required in both PCR and DNA replication
B) PCR is done in a tube while DNA replication occurs inside our cells
C) PCR and DNA replication both use a type of DNA ploymerase
D) PCR will always copy the entire chromosome
Chapter 5 Solutions
WHAT IS LIFE? A GUIDE TO BIO 3E+LAUNCHPA
Ch. 5 - Prob. 1SACh. 5 - Prob. 2SACh. 5 - Prob. 3SACh. 5 - Prob. 4SACh. 5 - Prob. 5SACh. 5 - Prob. 6SACh. 5 - Prob. 7SACh. 5 - Prob. 8SACh. 5 - Prob. 9SACh. 5 - Prob. 10SA
Ch. 5 - Prob. 11SACh. 5 - Prob. 12SACh. 5 - Prob. 13SACh. 5 - Prob. 14SACh. 5 - Prob. 15SACh. 5 - Prob. 1MCCh. 5 - Prob. 2MCCh. 5 - Prob. 3MCCh. 5 - Prob. 4MCCh. 5 - Prob. 5MCCh. 5 - Prob. 6MCCh. 5 - Prob. 7MCCh. 5 - Prob. 8MCCh. 5 - Prob. 9MCCh. 5 - Prob. 10MCCh. 5 - Prob. 11MCCh. 5 - Prob. 12MCCh. 5 - Prob. 13MCCh. 5 - Prob. 14MCCh. 5 - Prob. 15MCCh. 5 - Prob. 16MCCh. 5 - Prob. 17MC
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- Which of the following enzyme is required for end to end joining of DNA?a) DNA ligaseb) Restriction endonucleasec) RNA polymerased) DNA polymerasearrow_forwardWhich of the following enzymes has the highest functional error? a) DNA Polymerase I b) DNA Polymerase II c) DNA Polymerase III d) RNA Polymerase I e) RNA Polymerase IIarrow_forwardWhich of the following enzyme is used in PCR? a) EcoRII b) EcoRI c) Tag DNA polymerase d) HRParrow_forward
- What are the main components needed for electrophoresis? a) A power source b) A gel matrix c) A medium (or "buffer") d) Casting tray e) All of the above For what purpose is DNA electrophoresis used? a) To amplify a DNA for cloning b) To separate noncharged molecules of DNA based on size c) To separate charged molecules of DNA based on size d) To separate charged molecules of DNA based on amount of positive charge e) To produce static electricity by frictionarrow_forwardThe following statements are correct EXCEPT: A) Information in the DNA is transcribed int mRNA and translated into proteins B) Information in the DNA is translated int mRNA and transcribed into proteins C) Information in the DNA is copied by DNA polymerase D) The lagging strand during DNA replication is synthesized continuously to form the okazaki fragments E) B and Darrow_forwardThe technique of DNA sequencing involves performing _______________ . A) northern blotting B) Reverse transcription C) DNA replication D) electron microscopy of DNAarrow_forward
- Chemical mutagens often cause oxidation or deamination of DNA bases. This can lead to cancer by causing: A) Activation of replication. B) Activation of transcription. C) Non Watson-Crick base pairing. OD) Changes to cytochrome P450 enzymes. OE) Activation of translation.arrow_forwardWhat occurs during the HIGH temperature step of PCR? A) DNA is cut by restriction endonucleases B) primers get degraded C) primers anneal to single-stranded DNA D) DNA is synthesized E) double stranded DNA separates into single stranded DNAarrow_forwardYou discover an E. coli mutant that has a non-functional DNA polymerase III. Such a bactèria; a) Would not be able to synthesize new strands of DNA b) Would not be able synthesize the leading strand of DNA Oc) Would produce new strands of DNA still containing short RNA primers Od) Would be able to synthesize the lagging strand of DNA onlyarrow_forward
- Considering the Polymerase Chain Reaction (PCR), we can state that: a) It is a technique that promotes linear amplification of the number of DNA molecules in a sample. b) Depends on an initial template which can be a DNA or RNA molecule c) The reaction must contain, in addition to the template sequence, nitrogenous bases, sense and antisense primers and DNA polymerase enzyme. d)The primer sense rings on the template strand 3’>5’, extending from the 3’ end e)arrow_forwardWhich of the following ingredients does not belong in a PCR reaction? (A) DNA polymerase B Primer Ligase D) DNTPS E) ddNTPs F) Endonucleasearrow_forward8) Capillary electrophoresis involves a) Fragmentation of the DNA strands b) High resolution separation of fluorescently labelled DNA fragments c) Fluorescent activation of the DNA d) None of the above 9) Which of the following is not applicable to capillary electrophoresis? a) Multiplex PCR b) Forensic DNA profiling c) SNP genotyping d) Accurate DNA quantitationarrow_forward
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