Brock Biology of Microorganisms (15th Edition)
15th Edition
ISBN: 9780134261928
Author: Michael T. Madigan, Kelly S. Bender, Daniel H. Buckley, W. Matthew Sattley, David A. Stahl
Publisher: PEARSON
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Chapter 4.2, Problem 1MQ
- Approximately how large is the Escherichia coli genome in base pairs? How many genes does it contain?
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Approximately how large is the Escherichia coli genome in basepairs? How many genes does it contain?
The E. coli genome contains 1009 Chi sequences. Do these sequences occur at random, and, if not, how much more or less frequently than random do they occur?
The genome of D. melanogaster consists of approximately 1.7 * 108 base pairs. DNA synthesis occurs at a rate of 30 base pairs per second. In the early embryo, the entire genome is replicated in five minutes. How many bidirectional origins of synthesis are required to accomplish this feat?
Chapter 4 Solutions
Brock Biology of Microorganisms (15th Edition)
Ch. 4.1 - What is a genome and what is it composed of? What...Ch. 4.1 - Define the terms complementary and antiparallel as...Ch. 4.1 - Why is supercoiling essential to a bacterial cell?...Ch. 4.1 - Describe the central dogma of molecular biology....Ch. 4.2 - Approximately how large is the Escherichia coli...Ch. 4.2 - Prob. 2MQCh. 4.2 - Prob. 3MQCh. 4.2 - Prob. 1CRCh. 4.3 - Prob. 1MQCh. 4.3 - To which end (5 or 3) of a newly synthesized...
Ch. 4.3 - Prob. 3MQCh. 4.3 - What are the functions of DNA Pol I and III and...Ch. 4.3 - What is meant by the term semiconservative...Ch. 4.4 - Prob. 1MQCh. 4.4 - Prob. 2MQCh. 4.4 - Prob. 3MQCh. 4.4 - Prob. 1CRCh. 4.5 - What enzyme catalyzes transcription? What is a...Ch. 4.5 - Prob. 2MQCh. 4.5 - Prob. 3MQCh. 4.5 - Prob. 4MQCh. 4.5 - Prob. 1CRCh. 4.6 - What three major components make up an archaeal...Ch. 4.6 - Prob. 2MQCh. 4.6 - Prob. 3MQCh. 4.6 - How does the archaeal RNA polymerase differ from...Ch. 4.7 - Prob. 1MQCh. 4.7 - Differentiate between the different classes of...Ch. 4.7 - Prob. 3MQCh. 4.7 - Describe the two types of secondary structure a...Ch. 4.8 - Prob. 1MQCh. 4.8 - What is the function of the acceptor stem of a...Ch. 4.8 - Prob. 3MQCh. 4.8 - Prob. 1CRCh. 4.9 - Prob. 1MQCh. 4.9 - Prob. 2MQCh. 4.9 - Prob. 3MQCh. 4.9 - Why is the genetic code a degenerate code? What is...Ch. 4.10 - What are the components of a ribosome? What...Ch. 4.10 - How is a completed polypeptide chain released from...Ch. 4.10 - How does tmRNA free stalled ribosomes?Ch. 4.10 - Where on the ribosome do tRNAs bind, and what is...Ch. 4.11 - What are molecular chaperones and why are they...Ch. 4.11 - What macromolecules are protected by heat shock...Ch. 4.11 - How do chaperones assist the Escherichia coli cell...Ch. 4.11 - What proteins are involved in refolding misfolded...Ch. 4.12 - Prob. 1MQCh. 4.12 - Prob. 2MQCh. 4.12 - Prob. 3MQCh. 4.12 - Prob. 1CRCh. 4.13 - Prob. 1MQCh. 4.13 - Prob. 2MQCh. 4.13 - Prob. 3MQCh. 4.13 - Prob. 1CRCh. 4 - The genome of the bacterium Neisseria gonorrhoeae...Ch. 4 - Compare and contrast the activity of DNA and RNA...Ch. 4 - What would be the result (in terms of protein...
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- Neisseria gonorrhoeae what is the size mm (bx x 0.3 x 0.000001), how many base pairs does it have, how many encoding segments or genes, how many Non-encoding segements or pseudogenes, and how many structural RNAs?arrow_forwardRecombinant protein production by a genetically modified Escherichia coli strain is proportional to cell growth. Ammonia is used as a nitrogen source for aerobic glucose respiration. The recombinant protein has the general formula CH1,55O0,31N0,25, while that of the cellular biomass is CH1,77O0,49N0,24. The biomass yield from glucose equals 0.50 g/g, while the recombinant protein yield from glucose corresponds to 20% of the cell yield from substrate.a) How much ammonia is required? What is the oxygen demand? (b) If the biomass yield remains the same, what are the ammonia and oxygen requirements for a wild-type strain of E. coli, with cell biomass of the same elemental composition, but unable to synthesize the recombinant protein? (c) On an industrial scale, cultivation takes place in a continuous fermenter at 28°C and the desired recombinant protein production rate is 7 g/h. Since the viscosity of the culture broth is considerable, the energy input due to agitation cannot be neglected.…arrow_forwardYou have been tasked with designing a new computer algorithm to identify protein-coding genes in bacterial genomes.a) Name 3 specific criteria/patterns you will use to define whether a given sequence could contain a full-length protein-coding gene, and briefly justify your choices.b) Do you think your algorithm will work well to detect proteins encoded by the archaean Sulfolobus acidocaldarius? Why or why not?c) Do you think your algorithm will work well to detect proteins encoded in the eukaryotic yeast Saccharomyces cerevisiae? Why or why not?arrow_forward
- what are the genotypes of the following e.coli strains 1-4?arrow_forwardThe DNA of a deletion of alpha bacteriophage has a length of 15 micrometers instead of 17 micrometers? How many base pairs are missing from this mutant?arrow_forwardThe 13-Mb genome of the green alga Ostreococcus tauri contains ∼8000 genes. Compare the gene density in this eukaryote to that of E. coli (∼4300 genes) and that of A. thaliana (∼25,500 genes).arrow_forward
- On the gel diagram below, show how you believe these fragments will sort out during electrophoresis. Label each fragment with its correct number of base pairs. (8 fragments)arrow_forwardhow are okazaki fragments created?arrow_forwardhow will mutation (with - ) will affect E.coli grown in lactose medium. what is the implication of the following genotypes? i+ p+ o+ z- y+arrow_forward
- The unicellular, rod-shaped bacterium E. coli is~2 μm long and 0.8 μm wide, and has a genomeconsisting of a single 4.6 Mb circular DNA molecule. The unicellular archaean Methanosarcinaacetivorans is spherical (coccus-shaped) with adiameter of 3 μm and has a 5.7 Mb circular genome.The unicellular eukaryote Saccharomyces cerevisiaeis roughly spherical, with a diameter of 5–10 μm.It has a haploid genome of 12 Mb divided among16 linear chromosomes. Given these descriptions,how could you determine whether a new, uncharacterized microorganism was a bacterium, an archaean,or a eukaryote?arrow_forwardThe presence (+) or absence (−) of six sequences in each of five bacterial artificial chromosome (BAC) clones (A–E) is indicated in the following table. Using these markers, put the BAC clones in their correct order and indicate the locations of the numbered sequences within them. Sequences BAC clone 1 2 3 4 5 6 A + − − − + − B − − − + − + C − + + − − − D − − + − + − E + − − + − −arrow_forwardIt is desired to isolate genomic DNA from liquid culture of S. cerevisiae yeast. A commercial kit will be used to isolate genomic DNA from this liquid culture. Answer the following questions to understand the strategy used by commercial kits for genomic DNA isolation. a) List all the steps from cell pellet preparation to DNA elution. b) With which feature can the membrane in the column that comes with the commercial kit bind DNA? c) Which component in the kit would you use to recover the DNA from the membrane of the column to which the DNA was attached?arrow_forward
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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License