Concept explainers
To discuss:
Synthesis and regulation of non-protein molecules such as carbohydrates and steroids by the genes.
Introduction:
DNA is a genetic material consisting of a long stretch of
Want to see the full answer?
Check out a sample textbook solutionChapter 4 Solutions
CONNECT ACCESS CARD FOR ANATOMY AND PHYSIOLOGY
- a) Which of the following is not required for PCR? dNTPs bacterial plasmids carefully designed primers taq or other heat-resistant polymerases template DNA b) Sanger sequencing (aka the chain termination method) is a method for determining the sequence of nucleotides in a piece of DNA of interest requires use of special ddNTPs (dideoxy nucleotides) that have special fluorescent labels (dyes) attached to them and also lack a 3' hydroxyl requires fragments to be run through a tube containing a gel matrix where their fluorescent dyes are illuminated with a laser, which can be used to produce a chromatogram all of the above are true none of the above are true c) The technique that allows quick and efficient test-tube replication of DNA is: PCR Restriction enzyme analysis Dideoxy sequencing DNA gel electrophoresis Gene therapyarrow_forwarda) Use the figure below to answer the following question(s). The DNA profiles that follow represent four different individuals. Which of the following statements is most likely true regarding the results in the figure above? D is the child of A and C. D is the child of A and B. D is the child of B and C. A is the child of C and D. B is the child of A and C. b) A gel that matches the below picture could be created using which technologies in combination? microarray, PCR, gel electrophoresis vector cloning, dideoxy sequencing, gel electrophoresis STR analysis, dideoxy sequencing, gel electrophoresis PCR, dideoxy sequencing, STR analysis STR analysis, PCR, gel electorphoresisarrow_forwarda) Microarrays must be preceded by a Southern blot compare gene expression levels across many different genes in two different cell lines rely on the polar nature of the DNA molecule to separate these molecules based on size require months to run are rarely used in modern biotechnology b) CRISPR is a powerful gene editing tool because the programmable enzyme (Cas9) can find an exact sequence and make a cut at a precise nucleotide it can be applied to all types of cells it has both genetic editing and epigenetic functions it is inexpensive All of these are correct c) This person came up with the idea to use heat-resistant polymerases from volcanic oceanic vents during thermocycling in PCR reactions and later won a Nobel prize for this idea. Barbara McClintock George Beadle Jacob Monod Kary Mullis Francis Crickarrow_forward
- a) The word “cloning” refers to making copies of bacteria making cloned sheep or another eukaryotic organism copying a piece of DNA all of the above none of the above b) Which of these restriction enzymes will create a sticky end? an enzyme that has restriction site ATTA and cuts between the two T nucleotides an enzyme with restriction site AGTCAG and cuts between the T and C an enzyme with restriction site AATCGG and cuts between the A nucleotides. any non-palindromic nucleotide sequence used as a restriction site none of the above c) Which of the following do researchers not need to use during vector cloning? a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes restriction enzymes DNA polymerase a growth medium with carefully selected ingredients that take advantage of selectable markers. None of the abovearrow_forwardWhich of the following is a sequence most likely to be recognized by restriction enzymes? 5’ GATTAATC 3’- double-strand form 5’ ATG 3’- double-strand form 5’ AUGCGCAU 3’- double-strand form NH3-met-trp-val- COOHarrow_forwarda) An insecticidal protein that is now used widely as a pest prevention strategy in genetically engineered crops was first found in b) A medical researcher is trying to develop a diagnostic technique for a disease which is characterized by replication of a particular large chromosomal region. What would be the most appropriate technique? c) You are the scientific consultant for a television show about forensic analysis. In an upcoming episode, investigators will compare the DNA of a suspect and a DNA sample collected from the victim's fingernails. You are to write up a brief explanation of the technique required for comparing the DNA. Which technique should you explain?arrow_forward
- True or Falso a) ____Ti plasmid cloning requires the use of the Agrobacterium tumefaciens to insert a recombinant gene into a target plant. b) ____Vitamin A deficiency is a serious disease that can cause blindness and death in children, but genetic engineering has created a novel food product called golden rice that is fortified with Vitamin A and is freely available for use in rice-dependent countries order to combat this deficiency.arrow_forwardTrue or False? a.____If a restriction enzyme recognizes the restriction site, 5’AACGTT3’, and the enzyme cuts between the second A and the C, this will produce a “sticky end,” which is useful for cloning into a plasmid vector. b.____The DNA molecules will travel through the agar toward the negative end of the gel rig (black wires) during electrophoresis. c.____CRISPR was one of the first methods discovered in the 1970s for reliably amplifying gene products so that they can be sequenced and studied.arrow_forwarda) Describe the discovery of polymerase chain reaction (PCR), the components of the reaction and the generalized steps to the reaction b) Describe how restriction enzymes like EcoR1 are used to create recombinant plasmids and what the process is for using these plasmids to replicate a piece of target DNA. Include information about how to create sticky ends, the makeup of the bacterial plasmid and how to tell if the gene was successfully inserted in the plasmid and if the plasmid has been transformed by the bacteria. You may use a drawing to enhance your descriptionarrow_forward
- a) Long interspersed elements (LINEs) are a type of b) Sequences of DNA assembled by identifying overlaps among smaller DNA segments are known as c) Humans are diploid organisms, containing two sets of chromosomes. How many sets of chromosomes does wheat,Triticum aestivum, have? d) Your research group has recently determined the complete genomic sequence of the organism Examus biologisium. You instruct your group to begin sequence annotation, which involvesarrow_forwardTrue or False? a.____If a restriction enzyme recognizes the restriction site, 5’AACGTT3’, and the enzyme cuts between the second A and the C, this will produce a “sticky end,” which is useful for cloning into a plasmid vector. b.____The DNA molecules will travel through the agar toward the negative end of the gel rig (black wires) during electrophoresis. c.____CRISPR was one of the first methods discovered in the 1970s for reliably amplifying gene products so that they can be sequenced and studied.arrow_forwarda) Polymerase Chain Reaction is a way that scientists amplify (or create billions of copies of) DNA in vitro (in a tube.) b) Compare PCR with DNA Replication in the cell. Circle the components that are required for PCR. For the parts that are not circled, list them below and write an explanation for why they are no longer needed when DNA replication of a target gene is done in vitro. DNA Gyrase (or Topoisomerase) DNA polymerase Helicase SSBPs Ligase dNTPs Primers Sliding Clamp Replisomearrow_forward
- Human Heredity: Principles and Issues (MindTap Co...BiologyISBN:9781305251052Author:Michael CummingsPublisher:Cengage LearningBiology Today and Tomorrow without Physiology (Mi...BiologyISBN:9781305117396Author:Cecie Starr, Christine Evers, Lisa StarrPublisher:Cengage LearningBiology (MindTap Course List)BiologyISBN:9781337392938Author:Eldra Solomon, Charles Martin, Diana W. Martin, Linda R. BergPublisher:Cengage Learning