<LCPO> BIOLOGY
12th Edition
ISBN: 9781266216398
Author: Raven
Publisher: MCG CUSTOM
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Chapter 40.5, Problem 3LO
Summary Introduction
To describe: The three critical events that must accompany seed development.
Introduction: The plants are the multicellular eukaryotic organism in the kingdom Plantae. It is characterised by photosynthetic nutrition, a process through which it synthesis carbohydrates, essential unlimited growth at localised region, presence of cellulose in their cell wall and absence of locomotory organs.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 40 Solutions
<LCPO> BIOLOGY
Ch. 40.1 - Prob. 1LOCh. 40.1 - Prob. 2LOCh. 40.1 - Prob. 3LOCh. 40.2 - Name the four genetically regulated flowering...Ch. 40.2 - Define floral determination.Ch. 40.2 - Prob. 3LOCh. 40.3 - Prob. 1LOCh. 40.3 - Prob. 2LOCh. 40.3 - Prob. 3LOCh. 40.4 - Prob. 1LO
Ch. 40.4 - Prob. 2LOCh. 40.4 - Prob. 3LOCh. 40.5 - Prob. 1LOCh. 40.5 - Prob. 2LOCh. 40.5 - Prob. 3LOCh. 40.6 - Prob. 1LOCh. 40.6 - Prob. 2LOCh. 40.7 - Prob. 1LOCh. 40.7 - Prob. 2LOCh. 40.7 - Prob. 3LOCh. 40.8 - Prob. 1LOCh. 40.8 - Prob. 2LOCh. 40.8 - Prob. 3LOCh. 40 - Prob. 1DACh. 40 - Prob. 1IQCh. 40 - Prob. 2IQCh. 40 - Prob. 3IQCh. 40 - Morphogenesis is the development of a. growth...Ch. 40 - Vernalization induces flowering following exposure...Ch. 40 - Prob. 3UCh. 40 - Which of the following is NOT a component of a...Ch. 40 - Megasporcs are produced in a. anthers by mitosis....Ch. 40 - A stamen contains a a. style. b. stigma. c....Ch. 40 - Unlike bee-pollinated flowers, bird-pollinated...Ch. 40 - Prob. 8UCh. 40 - Endosperm is produced by the union of a. a central...Ch. 40 - During the globular stage of embryo development,...Ch. 40 - Which of the following is NOT a primary meristem?...Ch. 40 - Prob. 12UCh. 40 - The shoot tip of an emerging maize seedling is...Ch. 40 - Asexual reproduction is likely to be most common...Ch. 40 - Prob. 15UCh. 40 - Prob. 16UCh. 40 - Prob. 17UCh. 40 - Prob. 1ACh. 40 - Your roommate is taking biology with you this...Ch. 40 - In Iowa, a company called Team Corn works to...Ch. 40 - Prob. 4ACh. 40 - One of the most notable differences between gamete...Ch. 40 - A plant lacking the WOODEN LEG gene will likely a....Ch. 40 - How would plant development change if the...Ch. 40 - Prob. 8ACh. 40 - Loss-of-function mutations in the suspensor gene...Ch. 40 - Prob. 1SCh. 40 - If you live in a north temperate region, explain...Ch. 40 - In wild columbine flower morphology encourages...Ch. 40 - In most part of the world, commercial potato crops...Ch. 40 - Design an experiment to determine whether light or...
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forward
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