<LCPO> BIOLOGY
12th Edition
ISBN: 9781266216398
Author: Raven
Publisher: MCG CUSTOM
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Chapter 40, Problem 4A
Summary Introduction
Introduction:
The corn flowers are either staminate or carpellate flowers because of the maize is a monoecious plant. The development of four whorls of flowers is controlled by the homeotic genes.
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Look at the ideal results. Were your predictions accurate, and how did they compare with your results?
a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
Chapter 40 Solutions
<LCPO> BIOLOGY
Ch. 40.1 - Prob. 1LOCh. 40.1 - Prob. 2LOCh. 40.1 - Prob. 3LOCh. 40.2 - Name the four genetically regulated flowering...Ch. 40.2 - Define floral determination.Ch. 40.2 - Prob. 3LOCh. 40.3 - Prob. 1LOCh. 40.3 - Prob. 2LOCh. 40.3 - Prob. 3LOCh. 40.4 - Prob. 1LO
Ch. 40.4 - Prob. 2LOCh. 40.4 - Prob. 3LOCh. 40.5 - Prob. 1LOCh. 40.5 - Prob. 2LOCh. 40.5 - Prob. 3LOCh. 40.6 - Prob. 1LOCh. 40.6 - Prob. 2LOCh. 40.7 - Prob. 1LOCh. 40.7 - Prob. 2LOCh. 40.7 - Prob. 3LOCh. 40.8 - Prob. 1LOCh. 40.8 - Prob. 2LOCh. 40.8 - Prob. 3LOCh. 40 - Prob. 1DACh. 40 - Prob. 1IQCh. 40 - Prob. 2IQCh. 40 - Prob. 3IQCh. 40 - Morphogenesis is the development of a. growth...Ch. 40 - Vernalization induces flowering following exposure...Ch. 40 - Prob. 3UCh. 40 - Which of the following is NOT a component of a...Ch. 40 - Megasporcs are produced in a. anthers by mitosis....Ch. 40 - A stamen contains a a. style. b. stigma. c....Ch. 40 - Unlike bee-pollinated flowers, bird-pollinated...Ch. 40 - Prob. 8UCh. 40 - Endosperm is produced by the union of a. a central...Ch. 40 - During the globular stage of embryo development,...Ch. 40 - Which of the following is NOT a primary meristem?...Ch. 40 - Prob. 12UCh. 40 - The shoot tip of an emerging maize seedling is...Ch. 40 - Asexual reproduction is likely to be most common...Ch. 40 - Prob. 15UCh. 40 - Prob. 16UCh. 40 - Prob. 17UCh. 40 - Prob. 1ACh. 40 - Your roommate is taking biology with you this...Ch. 40 - In Iowa, a company called Team Corn works to...Ch. 40 - Prob. 4ACh. 40 - One of the most notable differences between gamete...Ch. 40 - A plant lacking the WOODEN LEG gene will likely a....Ch. 40 - How would plant development change if the...Ch. 40 - Prob. 8ACh. 40 - Loss-of-function mutations in the suspensor gene...Ch. 40 - Prob. 1SCh. 40 - If you live in a north temperate region, explain...Ch. 40 - In wild columbine flower morphology encourages...Ch. 40 - In most part of the world, commercial potato crops...Ch. 40 - Design an experiment to determine whether light or...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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- You will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. You have already cultured it and gone through the plate isolation procedure. Before you ship your samples off for sequencing, you want to do one final check of the A260 ratios. You get back the following ratios: A260/280 ratio is 1.89; A260/230 is 2.01. These ratios are close enough to the accepted "pure" values so they could be considered "pure" and mostly (if not completely) free of contaminants from the PCR process. True Falsearrow_forwardYou will use the following scenario to answer a group of 5 questions. You have isolated a microbe from an environmental sample. The microbe has the ability to perform a new metabolic reaction at a very low temperature, so you are excited that it could be a new species. After receiving your sequence back from the sequencing lab, you feel that you have, in fact, discovered and isolated a new species. You ask a fellow labmate about how you should proceed, and he tells you the following is the proper way to introduce a new species for recognition: Cultures have to be sent to international culture collections. Then a paper must be published describing the new organism and providing a genus and species name. You recall learning about this in your Microbiology course in college. Is this information from your colleague true or false? True Falsearrow_forwardis often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forward
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