<LCPO> BIOLOGY
12th Edition
ISBN: 9781266216398
Author: Raven
Publisher: MCG CUSTOM
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Chapter 40, Problem 2IQ
Summary Introduction
To determine: If the plants will flower at different times in the absence of flower-repressing genes and the vernalization and autonomous pathway genes induced expression of flower-promoting genes.
Introduction: The flowering plants have four different flowering pathways which are genetically regulated. The pathways include the light-dependent pathway, temperature pathway, gibberellin-dependent pathway, and the autonomous pathway.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 40 Solutions
<LCPO> BIOLOGY
Ch. 40.1 - Prob. 1LOCh. 40.1 - Prob. 2LOCh. 40.1 - Prob. 3LOCh. 40.2 - Name the four genetically regulated flowering...Ch. 40.2 - Define floral determination.Ch. 40.2 - Prob. 3LOCh. 40.3 - Prob. 1LOCh. 40.3 - Prob. 2LOCh. 40.3 - Prob. 3LOCh. 40.4 - Prob. 1LO
Ch. 40.4 - Prob. 2LOCh. 40.4 - Prob. 3LOCh. 40.5 - Prob. 1LOCh. 40.5 - Prob. 2LOCh. 40.5 - Prob. 3LOCh. 40.6 - Prob. 1LOCh. 40.6 - Prob. 2LOCh. 40.7 - Prob. 1LOCh. 40.7 - Prob. 2LOCh. 40.7 - Prob. 3LOCh. 40.8 - Prob. 1LOCh. 40.8 - Prob. 2LOCh. 40.8 - Prob. 3LOCh. 40 - Prob. 1DACh. 40 - Prob. 1IQCh. 40 - Prob. 2IQCh. 40 - Prob. 3IQCh. 40 - Morphogenesis is the development of a. growth...Ch. 40 - Vernalization induces flowering following exposure...Ch. 40 - Prob. 3UCh. 40 - Which of the following is NOT a component of a...Ch. 40 - Megasporcs are produced in a. anthers by mitosis....Ch. 40 - A stamen contains a a. style. b. stigma. c....Ch. 40 - Unlike bee-pollinated flowers, bird-pollinated...Ch. 40 - Prob. 8UCh. 40 - Endosperm is produced by the union of a. a central...Ch. 40 - During the globular stage of embryo development,...Ch. 40 - Which of the following is NOT a primary meristem?...Ch. 40 - Prob. 12UCh. 40 - The shoot tip of an emerging maize seedling is...Ch. 40 - Asexual reproduction is likely to be most common...Ch. 40 - Prob. 15UCh. 40 - Prob. 16UCh. 40 - Prob. 17UCh. 40 - Prob. 1ACh. 40 - Your roommate is taking biology with you this...Ch. 40 - In Iowa, a company called Team Corn works to...Ch. 40 - Prob. 4ACh. 40 - One of the most notable differences between gamete...Ch. 40 - A plant lacking the WOODEN LEG gene will likely a....Ch. 40 - How would plant development change if the...Ch. 40 - Prob. 8ACh. 40 - Loss-of-function mutations in the suspensor gene...Ch. 40 - Prob. 1SCh. 40 - If you live in a north temperate region, explain...Ch. 40 - In wild columbine flower morphology encourages...Ch. 40 - In most part of the world, commercial potato crops...Ch. 40 - Design an experiment to determine whether light or...
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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- is often a good indication of phylogenetic relatedness in phenotypes. Life-cycle patterns Cleavage patterns O Gene expression O Morphological similarityarrow_forwardWhich of the following is a weakness of using 16S rRNA for phylogenetic analyses? It can only go down to the family and genus levels It takes months to complete O Both of the above O None of the abovearrow_forwardAn unrooted tree containing ten unrelated species can become rooted by adding a descendant group related to two of the species. an unrelated outgroup. O a distantly related outgroup. O a descendant related to only one of the species.arrow_forward
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