<LCPO> BIOLOGY
12th Edition
ISBN: 9781266216398
Author: Raven
Publisher: MCG CUSTOM
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Chapter 40, Problem 12U
Summary Introduction
Introduction:
Germination of seed is the process through which a plant grows from a seed. Germination contains several stages, but it is characterized by the emergence of first root (radicle) from the seed. The embryo utilizes the food reserved in the seed and grows to form a living organism (plant).
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a) The lux operon is under positive control. Based on this information, does the luxR regulator sequence make a repressor protein or an activator protein?
b) How will binding of this complex affect RNA polymerase? Remember this operon is under positive control.
c) AHL is a signal molecule that V. fisheri makes to communicate with neighboring bacterial cells. This molecule can diffuse outside of the cell and into another bacterial cell in close proximity. This type of communication between bacterial cells is known as quorum sensing. If bacterial cell density is low how will this affect the lux operon? What will happen if the density is high?
1) Given an mRNA with the following sequence, please translate the codons to a chain of amino acids. Use the codon chart below.5’AUG/CCU/GCU/UAC/CGG/GAG/UAA3’
“met-_________-_________-_________-_________-_________”STOP”
2) Assuming the original polypeptide chain below, match each type of point mutation with the polypeptide chain that results.
Original polypeptide: Pro-Thr-Ser-Leu-Leu-His-Asn
A. Missense
B. Silent
C. Nonsense
D. Frameshift
______ Pro-Thr-Ser-STOP
______ Pro-Thr-Ser-Leu-Ile-His-Asn
______ Pro-Thr-Ser-Leu-Leu-His-Asn
_______ Pro-Thr-His-Cys-Tyr-Thr
Chapter 40 Solutions
<LCPO> BIOLOGY
Ch. 40.1 - Prob. 1LOCh. 40.1 - Prob. 2LOCh. 40.1 - Prob. 3LOCh. 40.2 - Name the four genetically regulated flowering...Ch. 40.2 - Define floral determination.Ch. 40.2 - Prob. 3LOCh. 40.3 - Prob. 1LOCh. 40.3 - Prob. 2LOCh. 40.3 - Prob. 3LOCh. 40.4 - Prob. 1LO
Ch. 40.4 - Prob. 2LOCh. 40.4 - Prob. 3LOCh. 40.5 - Prob. 1LOCh. 40.5 - Prob. 2LOCh. 40.5 - Prob. 3LOCh. 40.6 - Prob. 1LOCh. 40.6 - Prob. 2LOCh. 40.7 - Prob. 1LOCh. 40.7 - Prob. 2LOCh. 40.7 - Prob. 3LOCh. 40.8 - Prob. 1LOCh. 40.8 - Prob. 2LOCh. 40.8 - Prob. 3LOCh. 40 - Prob. 1DACh. 40 - Prob. 1IQCh. 40 - Prob. 2IQCh. 40 - Prob. 3IQCh. 40 - Morphogenesis is the development of a. growth...Ch. 40 - Vernalization induces flowering following exposure...Ch. 40 - Prob. 3UCh. 40 - Which of the following is NOT a component of a...Ch. 40 - Megasporcs are produced in a. anthers by mitosis....Ch. 40 - A stamen contains a a. style. b. stigma. c....Ch. 40 - Unlike bee-pollinated flowers, bird-pollinated...Ch. 40 - Prob. 8UCh. 40 - Endosperm is produced by the union of a. a central...Ch. 40 - During the globular stage of embryo development,...Ch. 40 - Which of the following is NOT a primary meristem?...Ch. 40 - Prob. 12UCh. 40 - The shoot tip of an emerging maize seedling is...Ch. 40 - Asexual reproduction is likely to be most common...Ch. 40 - Prob. 15UCh. 40 - Prob. 16UCh. 40 - Prob. 17UCh. 40 - Prob. 1ACh. 40 - Your roommate is taking biology with you this...Ch. 40 - In Iowa, a company called Team Corn works to...Ch. 40 - Prob. 4ACh. 40 - One of the most notable differences between gamete...Ch. 40 - A plant lacking the WOODEN LEG gene will likely a....Ch. 40 - How would plant development change if the...Ch. 40 - Prob. 8ACh. 40 - Loss-of-function mutations in the suspensor gene...Ch. 40 - Prob. 1SCh. 40 - If you live in a north temperate region, explain...Ch. 40 - In wild columbine flower morphology encourages...Ch. 40 - In most part of the world, commercial potato crops...Ch. 40 - Design an experiment to determine whether light or...
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- a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates? b) Predict the growth you would expect to see on each of the following plates: – DNA ___________________________________________________________ – DNA/+Amp ______________________________________________________ +DNA/+Amp ______________________________________________________ +DNA/+Amp/+IPTG _________________________________________________arrow_forward1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids? 2) Calculating Transformation Efficiency For the +DNA/+Amp/+IPTG plate, record the following: Number of transformants (colonies): _________________ Nanograms of plasmid DNA added: 50 ng Final recovery volume: 0.50 mL Volume plated: 0.25 mL Transformation efficiency equation: Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL) 3) Using the equation above, calculate the transformation efficiency. 4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forward
- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forwardIn oxidase test with Pseudomonas aeruginosa, the cell cultures on the slide turn colorless to be purple after tetra-methyl-p-phenylenediamine dihydrochloride (TMPD) is added. In the reaction, OTMPD is electron acceptor O cytochrome c is the electron source oxygen is terminal electron acceptor OH2 produced is electron donorarrow_forward
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