Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Chapter 38.2, Problem 3CC
WHAT IF? Two groups of individuals have CNS damage. In one group, the damage has resulted in a coma (a prolonged state of unconsciousness). In the other group, it has caused total paralysis (a loss of skeletal muscle function throughout the body). Relative to the position of the midbrain and pons, where is the likely site of damage in each group? Explain.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 38 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 38.1 - Which division of the autonomic nervous system...Ch. 38.1 - Prob. 2CCCh. 38.2 - When you wave your right hand, what part of your...Ch. 38.2 - People who are inebriated have difficulty touching...Ch. 38.2 - WHAT IF? Two groups of individuals have CNS...Ch. 38.3 - Prob. 1CCCh. 38.3 - Prob. 2CCCh. 38.3 - WHAT IF? If a woman with a severed corpus callosum...Ch. 38.4 - Which one of the five categories of sensory...Ch. 38.4 - Prob. 2CC
Ch. 38.4 - WHAT IF? If you stimulated a sensory neuron of an...Ch. 38.5 - Prob. 1CCCh. 38.5 - Prob. 2CCCh. 38.5 - Prob. 3CCCh. 38.5 - Prob. 4CCCh. 38.6 - Contrast the light-detecting organs of planarians...Ch. 38.6 - Prob. 2CCCh. 38.6 - MAKE CONNECTIONS Compare the function of retinal...Ch. 38 - Patients with damage to Wernickes area have...Ch. 38 - The cerebral cortex does not play a major role in...Ch. 38 - The middle ear converts A. air pressure waves to...Ch. 38 - Prob. 4TYUCh. 38 - Prob. 5TYUCh. 38 - Prob. 6TYUCh. 38 - Prob. 7TYUCh. 38 - SCIENTIFIC INQUIRY Consider an individual who had...Ch. 38 - Prob. 9TYUCh. 38 - FOCUS ON ORGANIZATION In a short essay (100-150...Ch. 38 - Prob. 11TYU
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- Overview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1) What differences would you expect to see between the…arrow_forwardOverview of Transformation Protocol -Prepare competent bacteria for transformation: Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure. Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed. -Transformation procedure: Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA. Add CaCl2 to both tubes. Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube. Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes. Add recovery broth to both tubes. Incubate both tubes in a 37 C water bath for 5 minutes. Questions: 1)What is the selectable marker in this experiment? How…arrow_forwardBased on your results, which suspect's DNA best matches the DNA found at the crime scene?arrow_forward
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