Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
2nd Edition
ISBN: 9780134433769
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman
Publisher: PEARSON
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Chapter 38.3, Problem 1CC
Summary Introduction
To determine:
The outline of two mechanisms by which information flow between two neurons in adults can increase.
Introduction:
Neuron is an excitable cell which receives the information, process it and transmit it through chemical and electrical signals. The connections present between neurons to transmit the information are known as synapsis. The neural network can be formed by connection of neurons which are prime component of CNS (central nervous system) including spinal cord and brain and of PNS (peripheral nervous system) comprise of somatic nervous system and autonomous nervous system.
There are mainly three types of specialized neurons sensory neuron, motor neuron and interneuron. Sensory neuron stimulates with only particular stimuli and the motor neuron receives signals from spinal cord and brain and conveys it to different parts of the body whereas interneuron connects neuron to neuron with brain or spinal cord.
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a) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
b) Using the equation above, calculate the transformation efficiency.
c) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
a) What differences would you expect to see between the -DNA/+Amp and +DNA/+Amp plates?
b) Predict the growth you would expect to see on each of the following plates:
– DNA ___________________________________________________________
– DNA/+Amp ______________________________________________________
+DNA/+Amp ______________________________________________________
+DNA/+Amp/+IPTG _________________________________________________
1)Which plate did you see purple/pink/blue bacterial cells? Why did you see this growth? Explain your answer in terms of transformation and plasmids?
2) Calculating Transformation Efficiency
For the +DNA/+Amp/+IPTG plate, record the following:
Number of transformants (colonies): _________________
Nanograms of plasmid DNA added: 50 ng
Final recovery volume: 0.50 mL
Volume plated: 0.25 mL
Transformation efficiency equation:
Transformation efficiency = Number of transformants / µg of DNA x Final volume at recovery (mL)/ volume plated (mL)
3) Using the equation above, calculate the transformation efficiency.
4) Describe the success of the transformation efficiency of this demo based on the calculation you did above?
Chapter 38 Solutions
Campbell Biology in Focus, Books a la Carte Edition; Modified Mastering Biology with Pearson eText - ValuePack Access Card - for Campbell Biology in Focus (2nd Edition)
Ch. 38.1 - Which division of the autonomic nervous system...Ch. 38.1 - Prob. 2CCCh. 38.2 - When you wave your right hand, what part of your...Ch. 38.2 - People who are inebriated have difficulty touching...Ch. 38.2 - WHAT IF? Two groups of individuals have CNS...Ch. 38.3 - Prob. 1CCCh. 38.3 - Prob. 2CCCh. 38.3 - WHAT IF? If a woman with a severed corpus callosum...Ch. 38.4 - Which one of the five categories of sensory...Ch. 38.4 - Prob. 2CC
Ch. 38.4 - WHAT IF? If you stimulated a sensory neuron of an...Ch. 38.5 - Prob. 1CCCh. 38.5 - Prob. 2CCCh. 38.5 - Prob. 3CCCh. 38.5 - Prob. 4CCCh. 38.6 - Contrast the light-detecting organs of planarians...Ch. 38.6 - Prob. 2CCCh. 38.6 - MAKE CONNECTIONS Compare the function of retinal...Ch. 38 - Patients with damage to Wernickes area have...Ch. 38 - The cerebral cortex does not play a major role in...Ch. 38 - The middle ear converts A. air pressure waves to...Ch. 38 - Prob. 4TYUCh. 38 - Prob. 5TYUCh. 38 - Prob. 6TYUCh. 38 - Prob. 7TYUCh. 38 - SCIENTIFIC INQUIRY Consider an individual who had...Ch. 38 - Prob. 9TYUCh. 38 - FOCUS ON ORGANIZATION In a short essay (100-150...Ch. 38 - Prob. 11TYU
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