Biology: Life on Earth with Physiology (11th Edition)
11th Edition
ISBN: 9780133923001
Author: Gerald Audesirk, Teresa Audesirk, Bruce E. Byers
Publisher: PEARSON
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Chapter 36, Problem 3RQ
Summary Introduction
To determine:
The role of
Introduction:
The nucleotides are the basic unit of genetic material of every organism. The nucleotides are made up of nitrogenous base, phosphate group and ribose sugar. The nucleotides play a vital role in the various functions of the body.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 36 Solutions
Biology: Life on Earth with Physiology (11th Edition)
Ch. 36.1 - Prob. 1CYLCh. 36.1 - define homeostasis and describe how urinary...Ch. 36.1 - Prob. 3CYLCh. 36.2 - describe and compare the urinary systems of...Ch. 36.3 - Prob. 1CSCCh. 36.3 - list and describe the structures of the human...Ch. 36.3 - Prob. 2CYLCh. 36.3 - Prob. 3CYLCh. 36.3 - draw and label a nephron?Ch. 36.4 - describe the blood supply of the nephron and how...
Ch. 36.4 - explain the three stages of urine formation,...Ch. 36.4 - describe where in the nephron each process occurs?Ch. 36.5 - Prob. 1CSCCh. 36.5 - Since the 1950s, when living kidney donation was...Ch. 36.5 - Prob. 1CYLCh. 36.5 - A patient on dialysis complains that she feels...Ch. 36.5 - Prob. 1HYEWCh. 36.5 - Prob. 1TCCh. 36.5 - explain the role of ADH in water reabsorption?Ch. 36.5 - What osmoregulatory problems would occur if a...Ch. 36.5 - Prob. 3CYLCh. 36.5 - Prob. 4CYLCh. 36.5 - explain how and why the urinary systems of...Ch. 36 - Prob. 1ACCh. 36 - Prob. 1FIBCh. 36 - Prob. 1MCCh. 36 - Explain the two major functions of urinary...Ch. 36 - Prob. 2ACCh. 36 - The human kidney consists of an outer layer called...Ch. 36 - Which of the following matches is correct? a....Ch. 36 - Prob. 2RQCh. 36 - Prob. 3FIBCh. 36 - Long nephron loops a. are common in animals with...Ch. 36 - Prob. 3RQCh. 36 - Prob. 4FIBCh. 36 - Which of the following is True? a. Collecting...Ch. 36 - Discuss the differences in function of the two...Ch. 36 - Fill in the following substances: produced from...Ch. 36 - Prob. 5MCCh. 36 - Describe the processes of filtration,...Ch. 36 - Prob. 6FIBCh. 36 - Describe the role of the kidneys as organs of...Ch. 36 - Prob. 7FIBCh. 36 - Briefly describe and compare the urinary systems...Ch. 36 - Explain and contrast osmoregulation in freshwater...
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