(a) Interpretation : The heaviest polypeptide chain at t=1 minute should be determined. Concept introduction : Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

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Answer 1

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Chapter 31, Problem 27P

Interpretation Introduction

(a)

Interpretation : The heaviest polypeptide chain at t=1 minute should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(b)

Interpretation : The order of labeling of peptides from the greatest to the least at t=3 minutes should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(c)

Interpretation:

The findings of experiment about protein synthesis should be determined.

Concept introduction:

Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 31, Problem 27P

Interpretation Introduction

(a)

Interpretation : The heaviest polypeptide chain at t=1 minute should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(b)

Interpretation : The order of labeling of peptides from the greatest to the least at t=3 minutes should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(c)

Interpretation:

The findings of experiment about protein synthesis should be determined.

Concept introduction:

Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

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Answer 3

Question

Chapter 31, Problem 27P

Interpretation Introduction

(a)

Interpretation : The heaviest polypeptide chain at t=1 minute should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(b)

Interpretation : The order of labeling of peptides from the greatest to the least at t=3 minutes should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(c)

Interpretation:

The findings of experiment about protein synthesis should be determined.

Concept introduction:

Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

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Answer 4

Question

Chapter 31, Problem 27P

Interpretation Introduction

(a)

Interpretation : The heaviest polypeptide chain at t=1 minute should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(b)

Interpretation : The order of labeling of peptides from the greatest to the least at t=3 minutes should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(c)

Interpretation:

The findings of experiment about protein synthesis should be determined.

Concept introduction:

Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

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Answer 5

Chapter 31, Problem 27P

Interpretation Introduction

(a)

Interpretation : The heaviest polypeptide chain at t=1 minute should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(b)

Interpretation : The order of labeling of peptides from the greatest to the least at t=3 minutes should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Interpretation Introduction

(c)

Interpretation:

The findings of experiment about protein synthesis should be determined.

Concept introduction:

Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Answer 6

Chapter 31, Problem 27P

Interpretation Introduction

(a)

Interpretation : The heaviest polypeptide chain at t=1 minute should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Answer 7

Chapter 31, Problem 27P

Interpretation Introduction

(a)

Interpretation : The heaviest polypeptide chain at t=1 minute should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Answer 8

Interpretation Introduction

(b)

Interpretation : The order of labeling of peptides from the greatest to the least at t=3 minutes should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Answer 9

Interpretation Introduction

(b)

Interpretation : The order of labeling of peptides from the greatest to the least at t=3 minutes should be determined.

Concept introduction :Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The findings of experiment about protein synthesis should be determined.

Concept introduction:

Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The findings of experiment about protein synthesis should be determined.

Concept introduction:

Protein synthesis (translation) is a process of generating new protein sequences inside the cell. This process takes place in the cytoplasm of the cell. This process is balanced by the degradation or export of cellular proteins. It is constituted of three steps namely, initiation, elongation, and termination.

Answer 12

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Answer 13

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Answer 14

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Answer 15

Ch. 31 - Prob. 1P Ch. 31 - Prob. 2P Ch. 31 - Prob. 3P Ch. 31 - Prob. 4P Ch. 31 - Prob. 5P Ch. 31 - Prob. 6P Ch. 31 - Prob. 7P Ch. 31 - Prob. 8P Ch. 31 - Prob. 9P Ch. 31 - Prob. 10P

Solution Summary: The author explains that protein synthesis (translation) is a process of generating new protein sequences inside the cell.

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