(a) Interpretation: Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined. Concept introduction: Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

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Answer 1

Question

Chapter 31, Problem 47P

Interpretation Introduction

(a)

Interpretation:

Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(b)

Interpretation:

The importance of measuring the helicase activity of eIF-4H as control should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(c)

Interpretation:

The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(d)

Interpretation:

The varying effect f eIF-4H with helix stability should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(e)

Interpretation:

The effect of eIF-4H on the helicase activity of eIF-4A should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 31, Problem 47P

Interpretation Introduction

(a)

Interpretation:

Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(b)

Interpretation:

The importance of measuring the helicase activity of eIF-4H as control should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(c)

Interpretation:

The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(d)

Interpretation:

The varying effect f eIF-4H with helix stability should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(e)

Interpretation:

The effect of eIF-4H on the helicase activity of eIF-4A should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

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Answer 3

Question

Chapter 31, Problem 47P

Interpretation Introduction

(a)

Interpretation:

Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(b)

Interpretation:

The importance of measuring the helicase activity of eIF-4H as control should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(c)

Interpretation:

The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(d)

Interpretation:

The varying effect f eIF-4H with helix stability should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(e)

Interpretation:

The effect of eIF-4H on the helicase activity of eIF-4A should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

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Answer 4

Question

Chapter 31, Problem 47P

Interpretation Introduction

(a)

Interpretation:

Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(b)

Interpretation:

The importance of measuring the helicase activity of eIF-4H as control should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(c)

Interpretation:

The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(d)

Interpretation:

The varying effect f eIF-4H with helix stability should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(e)

Interpretation:

The effect of eIF-4H on the helicase activity of eIF-4A should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

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Answer 5

Chapter 31, Problem 47P

Interpretation Introduction

(a)

Interpretation:

Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(b)

Interpretation:

The importance of measuring the helicase activity of eIF-4H as control should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(c)

Interpretation:

The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(d)

Interpretation:

The varying effect f eIF-4H with helix stability should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Interpretation Introduction

(e)

Interpretation:

The effect of eIF-4H on the helicase activity of eIF-4A should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 6

Chapter 31, Problem 47P

Interpretation Introduction

(a)

Interpretation:

Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 7

Chapter 31, Problem 47P

Interpretation Introduction

(a)

Interpretation:

Effects on eIF-4 helicase activity in the presence of eIF-4H should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The importance of measuring the helicase activity of eIF-4H as control should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The importance of measuring the helicase activity of eIF-4H as control should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The ratio of eIF-4H and eIF-4 should be determined to attain optimal activity.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 12

Interpretation Introduction

(d)

Interpretation:

The varying effect f eIF-4H with helix stability should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 13

Interpretation Introduction

(d)

Interpretation:

The varying effect f eIF-4H with helix stability should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of the eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 14

Interpretation Introduction

(e)

Interpretation:

The effect of eIF-4H on the helicase activity of eIF-4A should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 15

Interpretation Introduction

(e)

Interpretation:

The effect of eIF-4H on the helicase activity of eIF-4A should be determined.

Concept introduction:

Eukaryotic initiation factors (eIFs) are the proteins, which are used during the initiation of eukaryotic translation process. The function of these proteins is to stabilize the ribosomal complexes that are near the start codon on the mRNA strand.

Answer 16

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Answer 17

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Answer 18

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Answer 19

Ch. 31 - Prob. 1P Ch. 31 - Prob. 2P Ch. 31 - Prob. 3P Ch. 31 - Prob. 4P Ch. 31 - Prob. 5P Ch. 31 - Prob. 6P Ch. 31 - Prob. 7P Ch. 31 - Prob. 8P Ch. 31 - Prob. 9P Ch. 31 - Prob. 10P

Solution Summary: The author explains the importance of measuring the helicase activity of eIF-4H as control to attain optimal activity.

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Chapter 31 Solutions