Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 3, Problem 9R
Fill in the following table regarding the
| Appearance After This Step | |||
| Steps | Gram-Positive Cells | Gram-Negative Cells | |
| Crystal violet | a. __________ | e. __________ | |
| Iodine | b. __________ | f. __________ | |
| Alcohol-acetone | c. __________ | q. __________ | |
| Safranin | d. __________ | h. __________ | |
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Chapter 3 Solutions
Microbiology: An Introduction
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- Which of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.arrow_forwardExamine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information: A. Shape....... gram stain( +) , Gram (-) or both B. Stained color under microscope C. PG Wall thick, thin or botharrow_forwardThe acid-fast stain is used to stain _____. The decolorizer used to destain the cells is ______. Positive cells that retain the primary stain are ______, while negative cells are counterstained with _____ and will have a ______ color. *Please use answers from the options provided in the image below.arrow_forward
- How is the India ink capsule stain an example of a negative stain?arrow_forwardBoth crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forwardBacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorizationarrow_forward
- Explain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayarrow_forwardExamine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information A. Shape ........; Gram stain ( Gram (+), Gram (-) or both ... B. Stained color under microscope..... . C. Is the PG wall think, thin or both ..arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forward
- What is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedarrow_forwardBased from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:arrow_forwardWhat are the principle and basic concepts of GRAM STAIN? (please explain it thoroughly in a lengthy but clear explanation, thank you so much.)arrow_forward
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