Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Question
Chapter 3, Problem 2MCQ
Summary Introduction
Introduction:
Live cells can be visualized by various imaging techniques. One of the imaging methods commonly used to visualize live cells in a three-dimensional technology is by confocal microscopy.
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Shown below are several micrographs (images from microscopes) that were taken using different microscopy techniques. Write down what method of microscopy was used and why you thought so. Choices for microscopy techniques are: Transmitted Light Microscopy (techniques of brightfield, phase-contrast, darkfield, DIC all fall under this), Fluorescence Microscopy, Scanning Electron Microscopy, and Transmission Electron Microscopy.
Which of the follow are correct statements about microscope handling and care?
1. Always use TWO HANDS to carry the microscope.
2. Only use LENS PAPER to clean the lenses.
Never wipe back and forth, but simply make a gentle swipe across the lens.
3. A soft cloth should be used to clean the lenses.
4. Start and finish the day’s work at the LOWEST MAGNIFICATION.
5. Start and finish the day’s work at the HIGHEST MAGNIFICATION
6. Always BEGIN examining a specimen with a LOW POWER objective.
7. Always BEGIN examining a specimen with a HIGHEST POWER objective
8. On the compound microscope, NEVER use the coarse adjustment knob with the high power or oil objective.
9. On the compound microscope, ALWAYS use the coarse adjustment knob with the high power or oil objective.
10. Unplug the microscope by pulling on the plug, not the cord.
Chapter 3 Solutions
Microbiology: An Introduction
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- A. Purpose: Figure 1 B. Materials: Microscope Magazine Slides and cover slips Paper towels Pipette Scissors C. Procedure: 1. Careful carry a microscope to your lab area. Make sure to hold it with one hand under the base and one hand on the arm as shown in Figure 1. 2. Plug the microscope in and turn it on. Take a moment to look at all the parts of the microscope. Then look at your ocular lens. What is the magnification of the ocular lens (eye piece)? Figure 2 3. Fill in the chart to show the total magnification for each objective lens. Magnification of Ocular Lens Magnification of Objective Lens Objective Lens Total Magnification Low Power Medium Power High Powerarrow_forwarda) Briefly describe the concept of and instrument configuration for confocal microscopy. b) How do confocal and conventional microscopy compare? c) What other microscopy techniques can provide super-resolution?arrow_forwardDescribe 3 simple ways of preparing slides for light microscopy microscopearrow_forward
- 1. Match: Read about each microscope part. Match the description to the part on the diagram. Stage: Platform where a slide is placed. Eye piece: Lens at the top of the microscope that the user looks through. This lens most commonly magnifies a sample by 10x. Coarse focus knob: Large knob that moves the stage up and down to focus the sample. Fine focus knob: Small knob that moves the stage over a short distance to refine the focus. Objective lens: A second lens that further magnifies the sample. Microscopes usually have several objective lenses with different magnifications. Slide: A rectanqular piece of glass upon which a sample is mounted for viewing under a microscope.arrow_forwardList 6 ways on the proper handling/manipulation of the microscope.arrow_forwardHelp witharrow_forward
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