Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 3, Problem 6R
Why is a mordant used in the
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With what kind of gram stain do you use heat fixation? What is the purpose of heat fixation?
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Chapter 3 Solutions
Microbiology: An Introduction
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- What is the purpose of iodine in the gram staining process? Explain.arrow_forwardIs heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain procedure? Why or why not?arrow_forwardThis part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantarrow_forward
- Which step is the most crucial or most likely to cause poor results in the Gram stain?arrow_forwardWhat is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedarrow_forwardwhy does sometimes acid-fast bacteria accept the spore stain and spores accept the acid-fast stain.arrow_forward
- About the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?arrow_forwardWhat is the function of a mordant, and which reagent serves this purpose in the Gram stain procedure?arrow_forwardCan the gram stain be observed using a form of microscopy other than light microscopy? Why or whynot?arrow_forward
- Why is it essential that the primary stain and the counterstain be contrasting colors in gram-staining?arrow_forwardAdditional Differential Staining Procedures: Negative stain, Capsule Stain, Endospore stain, Flagella stain. What is a positive and negative results of this procedures?arrow_forwardwhy is it necessary to employ a spore stain rather than rely on diagnosis of sporing from examination of gram stainarrow_forward
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