Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 3, Problem 5MCQ
Which of the following is not a functionally analogous pair of stains?
- 1. nigrosin and malachite green
- 2. crystal violet and carbolfuchsin
- 3. safranin and methylene blue
- 4. ethanol-acetone and acid-alcohol
- 5. All of the above pairs are functionally analogous.
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Select all of the following that represent examples of positive stains.
Check All That Apply
nigrosin
methylene blue
crystal violet
India ink
safranin
What chemicals are used for the Simple Stain from our lab experience?
Check all that apply.
carbolic acid
mycolic acid
phenol
acid fuschin
malachite green
safranin
nigrosin
iodine
ethanol
methylene blue
crystal violet
carbol fuschin
Congo red
acid-alcohol
O O O O O O O
O O O O O O O
Different stains have different affinities for different organisms or different parts of organisms. Elaborate on this statement using the Hematoxylin and Eosin staining as an example.
Chapter 3 Solutions
Microbiology: An Introduction
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- List other fluorescent stains. What is the usefulness of each?arrow_forwardWhat is the effect when xylene is not removed when staining? What is/ are the specific action/s of the following stains on tissues? Hematoxylin Eosinarrow_forward1. Why must heat or a surface-active agent be used with application of the primary stain during acid-fast staining?2. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method. 3. What is the specific diagnostic value of this staining procedure?4. What is the specific diagnostic value of this staining procedure?5. Explain the importance of using methylene blue as the counterstain in the acid-fast staining method.6. What is the specific diagnostic value of acid fast staining procedure?arrow_forward
- For each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninarrow_forwardIodine in Ziehl Neelson’s staining serve as the mordant. True or false underline the word that is falsearrow_forwardWhich of the following is/are true regarding the acid-fast stain? (There may be more than one correct answer.) Non-acid-fast microbes appear blue in a completed acid-fast stain. O Acid-fast cells retain the primary dye after treatment with acid-alcohol. O It is used to identify members of the genus Mycobacterium. V Acid-fast cells appear bright pink/red in a completed acid-fast stain. O If cells are acid-fast, they are gram-negative.arrow_forward
- This part of the staining process helps the primary stain to remain in the cell during decolorization. O 1) Secondary stain O 2) primary stain O 3) counterstain O 4) fixing reagent O 5) mordantarrow_forward2) Assume that you made an error during your staining procedure. You used safranin as the primary stain and malachite green as counterstain. How would your cells appear at the end of the staining of the staining technique?arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forward
- What are Romanowsky stains? Name four of these.arrow_forwardChoose the 4 most correct steps from the list below for a simple positive stain and organize them into the most correct order. Briefly explain the purpose of each step you chose. • Heat fix • Dip slide in acid alcohol • Wash slide with ethanol • Apply Congo Red to the slide • Apply Crystal Violet to the slide • Make a smear • Wash slide with waterarrow_forwardAcid-Fast stain M. Smegmatis -morphology shape -Arrangement -Acid-fast or non-acid-fastarrow_forward
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