Biochemistry: Concepts and Connections
1st Edition
ISBN: 9780321839923
Author: Dean R. Appling, Spencer J. Anthony-Cahill, Christopher K. Mathews
Publisher: PEARSON
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Chapter 3, Problem 14P
Undergoing moderate activity, an average person will generate about 350 kJ of heat per hour. Using the heat of combustion of palmitic acid (
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The preferred substrate is T because the enzyme half-saturates at 7.00 mM for T, but requires 28.0 mM for U, and 112 mM for S.
b
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The rate constant k 2 with substrate S is 9.60×107 sec-1, with substrate T, k 2 = 6.00×104 sec-1, and with substrate U, k 2 = 2.40×106 sec-1.
Calculate the catalytic efficiency with S, T, and U.
Catalytic efficiency with S =
Catalytic efficiency with T =
Catalytic efficiency with U =
Does enzyme A use substrate S, substrate T, or substrate U with greater catalytic efficiency?
Fumerase catalyzes the conversion of fumerate to malate.
fumerate + H2O ⇋ malate
The turnover number, kcat, for fumerase is 8.00×102 sec-1. The Km of this enzyme for fumerate is 5.00×10-3 μmol mL-1.
a
In an experiment using 2.00×10-3 μmol·mL-1, what is Vmax?
Suppose you wanted to make a buffer of exactly pH 7.00 using KH2PO4 and Na2HPO4. If the final solution was 0.18 M in KH2PO4, you would need 0.25 M Na2HPO4. Now assume you wish to make a buffer at the same pH, using the same substances, but want the total phosphate molarity ([HPO42−]+[H2PO−4]) to equal 0.20 M. What concentration of the Na2HPO4 would be required?
Chapter 3 Solutions
Biochemistry: Concepts and Connections
Ch. 3 - Prob. 1PCh. 3 - Given the following reactions and their...Ch. 3 - The decomposition of crystalline N2O5...Ch. 3 - The oxidation of glucose to CO2 and water is a...Ch. 3 - Prob. 5PCh. 3 - In another key reaction in glycolysis,...Ch. 3 - Assume that some protein molecule, in its folded...Ch. 3 - When a hydrophobic substance like a hydrocarbon is...Ch. 3 - It is observed that as temperature is increased,...Ch. 3 - Suppose a reaction has Ho and So values...
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