To determine the five nonessential amino acids that can be biosynthesized using citric acid cycle intermediates. Concept introduction: Essential amino acids are those amino acids which cannot be synthesized by the body via biosynthesis and thus must be taken from the outside in form of dietary protein to meet the body’s need. Those amino acids which can be synthesized by biosynthesis within the liver are termed as nonessential amino acids. The starting materials required for the biosynthesis of 10 nonessential amino acids within the human body are 3-phosphoglycerate , pyruvate, oxaloacetate, α-ketoglutarate and phenylalanine.

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Biology You’re going to analyze 5 ul of your PCR product(out of 50 ul) on the gel. How much of 6X DNAloading buffer (dye) are you going to mix with yourPCR product to make final 1X concentration ofloading buffer in the PCR product-loading buffermixture?

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Chapter 26, Problem 26.90EP

Interpretation Introduction

Interpretation: To determine the five nonessential amino acids that can be biosynthesized using citric acid cycle intermediates.

Concept introduction: Essential amino acids are those amino acids which cannot be synthesized by the body via biosynthesis and thus must be taken from the outside in form of dietary protein to meet the body’s need. Those amino acids which can be synthesized by biosynthesis within the liver are termed as nonessential amino acids.

The starting materials required for the biosynthesis of 10 nonessential amino acids within the human body are 3-phosphoglycerate, pyruvate, oxaloacetate, α-ketoglutarate and phenylalanine.

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