FUNDAMENTALS OF BIOCHEMISTRY - LL FD
5th Edition
ISBN: 9781119598022
Author: Voet
Publisher: WILEY
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Chapter 26, Problem 15E
Summary Introduction
To explain: The reason why the 02- methylation of ribose residues protects rRNA from RNases.
Introduction: Modifications of rRNA are concentrated in the functional regions such as peptidyl transferase center. These modifications are necessary for the ribosome maturation and any alteration in these modifications can cause cellular defects and diseases. The presence of 2'-O- methyl in ribose residues prevents the hydrolysis of the phosphate present in the backbone and encourages the ribose sugar to make a 3'-endo conformation.
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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 26 Solutions
FUNDAMENTALS OF BIOCHEMISTRY - LL FD
Ch. 26 - Prob. 1ECh. 26 - 2. The bacterial enzyme polynucleotide...Ch. 26 - Prob. 3ECh. 26 - Prob. 4ECh. 26 - Prob. 5ECh. 26 - Prob. 6ECh. 26 - Prob. 7ECh. 26 - Prob. 8ECh. 26 - Prob. 9ECh. 26 - 10. Certain E. coli bacteriophages encode a...
Ch. 26 - Prob. 11ECh. 26 - Prob. 12ECh. 26 - 13. Compare DNA polymerase, RNA polymerase, poly...Ch. 26 - Prob. 14ECh. 26 - Prob. 15ECh. 26 - Prob. 16ECh. 26 - Prob. 17ECh. 26 - Prob. 18ECh. 26 - Prob. 19ECh. 26 - Prob. 20ECh. 26 - Prob. 21ECh. 26 - Prob. 22ECh. 26 - Prob. 23CQCh. 26 - Prob. 24CQCh. 26 - Prob. 25CQCh. 26 - Prob. 26CQCh. 26 - Prob. 27CQCh. 26 - Prob. 28CQCh. 26 - Prob. 29CQCh. 26 - Prob. 30CQCh. 26 - Prob. 1MTE
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