CONNECT IA GENERAL ORGANIC&BIO CHEMISTRY
4th Edition
ISBN: 9781260562620
Author: SMITH
Publisher: MCG
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Chapter 22, Problem 71P
Interpretation Introduction
Interpretation:
The sticky ends that result from cleavage of the double stranded DNA segment with BamH1 should be drawn.
Concept Introduction:
Restriction endonucleases recognizes a specific
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Q23:
Dr. Alghe has just performed a restriction digest on DNA she extracted from her samples. She must now run her DNA out on a gel to separate the fragments based on size. To do this she must prepare a 1.1% agarose gel, where the percentage is determined as mass/vol.
How many grams of agarose must she add to 413 mL of buffer in order to arrive at the correct percentage? (answer to 2 decimals)
**Assume the dissolution of agarose will not alter the final volume of the solution**
During base excision repair, DNA glycosylase cleaves theN-glycosidic link between the altered base and the deoxyribose component of the nucleotide. Draw a typical nucleotideand indicate which bond is cleaved.
Protein synthesis begins when a ribosome assembles at an initiation codon (AUG) in the mRNA.
The completed protein chain is released from the ribosome when an initiation codon (UAA, UAG, or UGA) is reached
a. Both of the statements are TRUE
b. Both of the statements are FALSE
c. Statement I is TRUE, Statement II is FALSE
d. Statement I is FALSE, Statement II is TRUE
Chapter 22 Solutions
CONNECT IA GENERAL ORGANIC&BIO CHEMISTRY
Ch. 22.1 - Prob. 22.1PPCh. 22.1 - Prob. 22.1PCh. 22.1 - -Fluorouracil is an anticancer drug that...Ch. 22.1 - Prob. 22.3PCh. 22.1 - Prob. 22.4PCh. 22.1 - Label each statement about the compound...Ch. 22.1 - Draw the structure of each nucleotide: (a) UMP;...Ch. 22.1 - Give the name that corresponds to each...Ch. 22.2 - Draw the structure of a dinucleotide formed by...Ch. 22.2 - Draw the structure of each polynucleotide: (a) CU;...
Ch. 22.2 - Label each statement about the polynucleotide...Ch. 22.3 - Write the complementary strand for each of the...Ch. 22.4 - What is the sequence of a newly synthesized DNA...Ch. 22.6 - For each DNA segment: [1] What is the sequence of...Ch. 22.6 - Prob. 22.9PCh. 22.7 - What amino acid is coded for by each codon? GCC...Ch. 22.7 - What codons code for each amino acid? a. glycine...Ch. 22.7 - Drive the amino acid sequence that is coded for by...Ch. 22.7 - Write a possible mRNA sequence that codes for each...Ch. 22.7 - Considering the given sequence of nucleotides in...Ch. 22.8 - Prob. 22.14PCh. 22.8 - Prob. 22.8PPCh. 22.8 - Prob. 22.9PPCh. 22.9 - Prob. 22.10PPCh. 22.9 - Prob. 22.15PCh. 22.10 - Prob. 22.16PCh. 22 - Label each statement as pertaining to DNA, RNA, or...Ch. 22 - Label each statement as pertaining to DNA, RNA, or...Ch. 22 - Prob. 19PCh. 22 - (a) Give the name of each compound shown as a...Ch. 22 - Prob. 21PCh. 22 - Prob. 22PCh. 22 - Prob. 23PCh. 22 - Prob. 24PCh. 22 - Prob. 25PCh. 22 - Draw the structure of each of the following: a...Ch. 22 - Prob. 27PCh. 22 - Prob. 28PCh. 22 - Prob. 29PCh. 22 - Prob. 30PCh. 22 - Prob. 31PCh. 22 - Draw the structures of the two possible...Ch. 22 - Prob. 33PCh. 22 - Draw the structure of each dinucleotide and...Ch. 22 - Draw the deoxyribonucleotide TGA. Label the 5 and...Ch. 22 - Draw the ribonucleotide CGU. Label the 5 and 3...Ch. 22 - Prob. 37PCh. 22 - Describe in detail the DNA double helix with...Ch. 22 - Write the sequence of the complementary strand of...Ch. 22 - Prob. 40PCh. 22 - Prob. 41PCh. 22 - Prob. 42PCh. 22 - Prob. 43PCh. 22 - Prob. 44PCh. 22 - Prob. 45PCh. 22 - Figure 22.4 snows the hydrogen-bonding...Ch. 22 - Prob. 47PCh. 22 - Prob. 48PCh. 22 - Prob. 49PCh. 22 - Prob. 50PCh. 22 - What mRNA is transcribed from each DNA sequence in...Ch. 22 - Prob. 52PCh. 22 - For each DNA segment: [1] What is the sequence of...Ch. 22 - Prob. 54PCh. 22 - For each codon, give its anticodon and the amino...Ch. 22 - For each codon, give its anticodon and the amino...Ch. 22 - Fill in the missing information in the schematic...Ch. 22 - Fill in the missing information in the schematic...Ch. 22 - Derive the amino acid sequence that is coded for...Ch. 22 - Derive the amino acid sequence that is coded for...Ch. 22 - Write a possible mRNA sequence that codes for each...Ch. 22 - Prob. 62PCh. 22 - Prob. 63PCh. 22 - Prob. 64PCh. 22 - Prob. 65PCh. 22 - Prob. 66PCh. 22 - Consider the following mRNA sequence:...Ch. 22 - Consider the following mRNA sequence: 5-ACC UUA...Ch. 22 - Consider the following sequence of DNA: 3-TTA...Ch. 22 - Consider the following sequence of DNA: 3-ATA...Ch. 22 - Prob. 71PCh. 22 - Prob. 72PCh. 22 - Prob. 73PCh. 22 - Prob. 74PCh. 22 - Prob. 75PCh. 22 - Prob. 76PCh. 22 - Prob. 77PCh. 22 - Prob. 78PCh. 22 - Prob. 79PCh. 22 - Prob. 80PCh. 22 - Prob. 81PCh. 22 - Prob. 82PCh. 22 - Fill in the base, codon, anticodon, or amino acid...Ch. 22 - Fill in the base, codon, anticodon, or amino acid...Ch. 22 - Fill in the base, codon, anticodon, or amino acid...Ch. 22 - Prob. 86PCh. 22 - Prob. 87PCh. 22 - Prob. 88PCh. 22 - Prob. 89PCh. 22 - Prob. 90PCh. 22 - Prob. 91CPCh. 22 - Give a possible nucleotide sequence in the...
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- In the context of DNA replication, what is the difference between a leading DNA strand and a lagging DNA strand?arrow_forwardThe -chain of hemoglobin is a protein that contains 146 amino acids residues. What minimum number of nucleotides must be present in a strand of mRNA that is coded for this protein?arrow_forwardb) Suppose a mutation in an enzyme resulted in the replacement of a leucine with a serine in the active site of the enzyme (so you have a serine where you should have a leucine). How might that affect substrate binding?arrow_forward
- Here is a portion of the nucleotide sequence in phenylalanine tRNA. 3'-ACCACCUGCUCAGGCCUU-5' Write the nucleotide sequence of its DNA complementarrow_forwardRetroviruses like HIV, the pathogen responsible for AIDS, incorporate an RNA template that is copied into DNA duringinfection. The reverse transcriptase enzyme that copies RNA into DNA is relatively nonselective and error-prone, leadingto a high mutation rate. Its lack of selectivity is exploited by the anti-HIV drug AZT (3′@azido@2′,3′@dideoxythymidine),which becomes phosphorylated and is incorporated by reverse transcriptase into DNA, where it acts as a chain terminator.Mammalian DNA polymerases are more selective, having a low affinity for AZT, so its toxicity is relatively low.(a) Draw the structures of AZT and natural deoxythymidine.(b) Draw the structure of AZT 5′@triphosphate, the derivative that inhibits reverse transcriptase.*2arrow_forwardWhich statement BEST describes why the sugar phosphate nucleic acid backbone is on the outer part of the DNA double helix while the nitrogenous bases are on the inside of the double helix? O The sugar phosphate backbone is hydrophilic while the bases are less polar. ○ The sugar phosphate backbone is hydrophobic while the bases are hydrophilic. The nitrogen bases are covalently bonded to each other. O There is no reason; the structure is just that way.arrow_forward
- For the polymerase chain reaction to occur, ____. a. a large sample of DNA is needed b. a sticky end must be available for the ligase enzyme to function c. the DNA must remain double stranded d. the reaction temperature must be cycled from low to high e. all DNA fragments must be identicalarrow_forwardtemperature of 45 °C for 15 seconds in a PCR reaction is needed for A The two strands of DNA open B. The primers bind to the ends of the gene to be amplified C. polymerase can bind D. The strand is extended at temperatures that do not damage DNA Explain your answer with explanationarrow_forwardReplication of DNA produces two daughter DNA molecules in which a. once daughter molecules contains both parent strands and one daughter molecule contains both newly synthesized strands b. each daughter molecule contians one parent strand and one newly synthesized strand c. each daughter molecule contians two newly synthesized strands d. each daughter molecule contians both parent strandsarrow_forward
- During DNA replication, which of the following enzymes acts as the "workhorse," inserting DNA nucleotides to create new strands of DNA? O a. Ligase O b. DNA Polymerase III O c. Primase Od. Helicase e. DNA Polymerase Iarrow_forwardDNA Replication1) Construct the complementary strand of DNA (strand II) that would pair with the DNA strand I sequence below. Remember that nucleotides are always added in the 5 prime to 3 prime direction. The base pairing rules are: DNA DNAadenine (A) always bonds with thymine (T) thymine (T) always bonds with adenine (A) guanine (G) always bonds with cytosine (C) cytosine (C) always bonds with guanine (G) STRAND I: 3’ T A C T A T A G T C T G T C T C C C A C T 5’ STRAND II: 5’ 3’arrow_forwardIf PCR and DNA replication are very synonymous processes, what components are lacking in PCR requiring it to have varying temperatures whereas DNA replication may occur in normal physiological temperatures?arrow_forward
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