Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN: 9781305389892
Author: Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher: Cengage Learning
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Textbook Question
Chapter 22, Problem 3TYK
A characteristic that exhibits smooth changes in populations distributed along a geographical gradient is called a:
a. ring species.
b. hybrid.
c. cline.
d. hybrid breakdown.
e. subspecies.
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Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
Questions:
1) What differences would you expect to see between the…
Overview of Transformation Protocol
-Prepare competent bacteria for transformation:
Treat starter E. coli bacteria with CaCl2and Competent Cell Solution (CCS). Store on ice until transformation procedure.
Competent cells are cells that are likely to take up foreign DNA and be transformed. This step increases the likelihood that the E. coli cells will take up the introduced vector and be transformed.
-Transformation procedure:
Obtain two microcentrifuge tubes containing your competent cells. Label one tube +DNA and one -DNA.
Add CaCl2 to both tubes.
Add the transformation mix containing the plasmid DNA to the tube labeled +DNA. Do not add any plasmid DNA to the -DNA tube.
Incubate both tubes on ice for 10 minutes. Then, place both tubes in a 42\deg C water bath for 45 seconds. Replace the tubes in an ice bucket for 2 minutes.
Add recovery broth to both tubes.
Incubate both tubes in a 37 C water bath for 5 minutes.
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1)What is the selectable marker in this experiment? How…
Based on your results, which suspect's DNA best matches the DNA found at the crime scene?
Chapter 22 Solutions
Biology: The Dynamic Science (MindTap Course List)
Ch. 22.1 - How do the morphological, biological, and...Ch. 22.1 - What is clinical variation?Ch. 22.2 - What is the difference between prezygotic and...Ch. 22.2 - Prob. 2SBCh. 22.3 - Prob. 1SBCh. 22.3 - Why might insects from different host races be...Ch. 22.4 - Prob. 1SBCh. 22.4 - Prob. 2SBCh. 22 - The biological species concept defines species on...Ch. 22 - Prob. 2TYK
Ch. 22 - A characteristic that exhibits smooth changes in...Ch. 22 - Prezygotic is isolating mechanisms: a. reduce the...Ch. 22 - In the model of allopatric speciation, the...Ch. 22 - Prob. 6TYKCh. 22 - If two species of holly (genus Ilex) flower during...Ch. 22 - Prob. 8TYKCh. 22 - An animal breeder, attempting to cross a llama...Ch. 22 - Prob. 10TYKCh. 22 - Apply Evolutionary Thinking How do human...Ch. 22 - Prob. 12TYKCh. 22 - Prob. 13TYKCh. 22 - Prob. 14TYKCh. 22 - Prob. 15TYKCh. 22 - David Hills of Baylor University noted that three...
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