Chapter 22, Problem 22.29P

Interpretation Introduction

Interpretation:

Concentrations of nitrite and nitrate in the urine sample have to be determined.

Concept Introduction:

If the $\text{N}$ internal standard undergo the same reaction and same separation at the same rate as the $\text{N}$ analytes, then the concentration of analysis are simply calculated as follows:

    $\text{Concentration}\text{of}\text{N}{\text{O}}_x^{-}=\left(\text{Concentration}\text{of}\text{N}{\text{O}}_x^{-}\right)\left(\text{R}-{\text{R}}_{\text{Blank}}\right)$

Here, R is the measured ratio of  peak area of the analyte.

${\text{R}}_{\text{Blank}}$ is the measured ratio of peak areas from the same buffers and reagents with no added nitrate or nitrite.