FUNDAMENTALS OF BIOCHEMISTRY - LL FD
5th Edition
ISBN: 9781119598022
Author: Voet
Publisher: WILEY
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Chapter 22, Problem 21E
Summary Introduction
To explain: The reason why type 1 diabetes requires the insulin injections, whereas it is effective only in a portion of type-2 diabetes.
Concept introduction: Diabetes mellitus usually known as diabetes is a group of
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Students have asked these similar questions
The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism
of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help
To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?
To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine.
Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!
Chapter 22 Solutions
FUNDAMENTALS OF BIOCHEMISTRY - LL FD
Ch. 22 - Prob. 1ECh. 22 - Prob. 2ECh. 22 - 3. Toxins that occur in certain mushrooms block ?...Ch. 22 - Prob. 4ECh. 22 - 5. The passive glucose transporter named GLUT1...Ch. 22 - Prob. 6ECh. 22 - Prob. 7ECh. 22 - 8. (a) Identify the two reactions that allow the...Ch. 22 - Prob. 9ECh. 22 - Prob. 10E
Ch. 22 - Prob. 11ECh. 22 - 12. Predict the effect of an overdose of insulin...Ch. 22 - 13. Would you expect insulin to increase or...Ch. 22 - Prob. 14ECh. 22 - 15. Why can adipose tissue be considered to be an...Ch. 22 - 16. If the circulatory system of an ob/ob mouse is...Ch. 22 - 17. In experiments to test the...Ch. 22 - Prob. 18ECh. 22 - Prob. 19ECh. 22 - Prob. 20ECh. 22 - Prob. 22ECh. 22 - Prob. 21ECh. 22 - 23. AMPK activates phosphofructokinase-2. Explain...Ch. 22 - Prob. 24ECh. 22 - Prob. 25ECh. 22 - Prob. 26ECh. 22 - 27. Why does oxidative metabolism, which generates...Ch. 22 - Prob. 28CQCh. 22 - 29. Pancreatic ? cells express a receptor for...Ch. 22 - Prob. 30CQCh. 22 - Prob. 31CQCh. 22 - Prob. 32CQCh. 22 - 33. Experienced runners know that it is poor...Ch. 22 - 34. After several days of starvation, the capacity...Ch. 22 - Prob. 35CQCh. 22 - Prob. 36CQCh. 22 - Prob. 37CQCh. 22 - Prob. 38CQCh. 22 - Prob. 39CQCh. 22 - Prob. 40CQCh. 22 - Prob. 1MTE
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