(a) Interpretation: The reason the proteins are not visible in the lanes without amylase treatment should be discussed. Concept introduction: Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules. SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 1

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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The beta-lactamase hydrolyzes the lactam-ring in penicillin. Describe the mechanism of hydrolysis, insuring to include the involvement of S, D, & K in the reaction sequence. Please help

To map the active site of beta-lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Why doesn't D in this hexapeptide not participate in the hydrolysis of the beta-lactam ring even though S, K, and D are involved in the catalyst?

To map the active site of -lactamase, the enzyme was hydrolyzed with trypsin to yield a hexapeptide (P1) with the following amino acids. Glu, Lys, Leu, Phe, Met, and Ser. Treatment of P1 with phenyl isothiocyanate yielded a PTH derivative of phenylalanine and a peptide (P2). Treatment of P1 with cyanogenbromide gave an acidic tetrapeptide (P3) and a dipeptide (P4).Treatment of P2 with 1-fluoro-2,4-dinitrobenzene, followed by complete hydrolysis, yields N-2,4-dinitrophenyl-Glu. P1, P2, and P3 contain the active site serine. Using the experimental results described above derive the primary sequence of the active site hexapeptide. Please help!

Answer 2

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 3

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 4

Question

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

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Answer 5

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 6

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 7

Chapter 21, Problem 43P

Interpretation Introduction

(a)

Interpretation:

The reason the proteins are not visible in the lanes without amylase treatment should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The effect of treating the sample with a-amylase should be explained.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The list of other glycogen associated proteins and the reasons for their absence in SDS-PAGE should be discussed.

Concept introduction:

Alpha-amylase catalyzes the hydrolysis of alpha linkages in polysaccharides and release glucose and maltose. Glycogenin is an enzyme which catalyzes the formation of short glycogen primers from glucose molecules.

SDS-PAGE is a biochemical technique used to separate charge molecules in a mixture, according to their molecular masses. SDS acts as a surfactant and gives negative charge to the proteins. Thus it allows the protein separation only based on their molecular mass. Western blot is a visualizing technique which is commonly used to analyze proteins in a given sample via SDS-PAGE.

Answer 12

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Answer 15

Ch. 21 - Prob. 1P Ch. 21 - Prob. 2P Ch. 21 - Prob. 3P Ch. 21 - Prob. 4P Ch. 21 - Prob. 5P Ch. 21 - Prob. 6P Ch. 21 - Prob. 7P Ch. 21 - Prob. 8P Ch. 21 - Prob. 9P Ch. 21 - Prob. 10P

Solution Summary: The author explains that the proteins are not visible in the lanes without amylase treatment.

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