PRESCOTT'S MICROBIOLOGY
11th Edition
ISBN: 2818440045677
Author: WILLEY
Publisher: MCG
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Chapter 20.3, Problem 2CC
Summary Introduction
Microorganisms generating methane as a
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Consider the following equation: Nt = N0ert 1. What type of growth is modelled by this equation?
Asap explain well
Theoretical Data
Following the modified protocol for the isolation of Escherichia coli bacteriophage of Encabo (2018)
presented below, compute for the pfu/ml of the chloroform-treated lysate.
1ml
1ml
1ml 1ml
1
Chloroform-treated lysate
1ml
Dilution
10-3
10-4
10-5
0 0 0 0 0
9ml
9ml 9ml
10-1
10-²
10-3
Empty sterile
tubes
Incubate inside ref for 15-20min
+ 0.1 ml lysate-E.coli mix
0.1 ml
9ml 9ml
104
10.5
//
0.1 ml
0.1 ml
+0.5 ml E. coli
↓↓↓↓↓↓
Incubate O/N at 35°C,
observe for plaques
Molten soft agar
overlay
B. Quantification of the Infective E. coli other Bacteriophage Particles in the Raw Sewage
Sample
Volume of Stock Plated (in ml)
Bottom agar
No. of Plaque-Forming Units (pfu)
Ave.
A
B
254
265
132
110
11
23
Chapter 20 Solutions
PRESCOTT'S MICROBIOLOGY
Ch. 20.1 - MICRO INQUIRY Given that the DC/HB pathway uses...Ch. 20.1 - Prob. 2MICh. 20.1 - Prob. 1CCCh. 20.1 - Why do methanogens use the reductive acetyl-CoA...Ch. 20.1 - Prob. 3CCCh. 20.1 - Compare the ATP and NADH yield of each of the...Ch. 20.2 - MICRO INQUIRY Identify the potential electron...Ch. 20.2 - Retrieve, Infer, Apply What are thermoacidophiles...Ch. 20.2 - Prob. 2CCCh. 20.2 - Compare the carbon and sulfur metabolism of...
Ch. 20.2 - Prob. 4CCCh. 20.2 - Prob. 5CCCh. 20.3 - MICRO INQUIRY What are the mechanisms by which...Ch. 20.3 - Retrieve, Infer, Apply Why are the growth yields...Ch. 20.3 - Prob. 2CCCh. 20.3 - What is the ecological and practical importance of...Ch. 20.3 - Where are extreme halophiles found, and what is...Ch. 20.3 - What is the difference between sensory rhodopsin...Ch. 20.3 - How is Thermoplasma able to live in acidic, very...Ch. 20.3 - In what way are members of the genus Archaeoglobus...Ch. 20 - Prob. 1RCCh. 20 - Prob. 2RCCh. 20 - Prob. 3RCCh. 20 - Prob. 4RCCh. 20 - Prob. 5RCCh. 20 - Prob. 6RCCh. 20 - Prob. 7RCCh. 20 - Some believe that archaea should not be separate...Ch. 20 - Prob. 2ALCh. 20 - Prob. 3ALCh. 20 - Prob. 4ALCh. 20 - Prob. 5ALCh. 20 - Prob. 6ALCh. 20 - Prob. 7AL
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