To determine: The reason why the expression of mcrA transcripts is a good measure of methane production.
Introduction: The prokaryotes which produce methane are a group of archaea. The members of archaea, reduce carbon dioxide to produce methane and are known as methanogens. The methanogens reduce carbon dioxide with the help of hydrogen in the process of fermentation.
To determine: The taxonomic order, which is most likely prevalent in that particular environment.
Introduction: Methanogens are mostly found in anaerobic environments. They thrive in the extreme conditions in which the electron acceptors can be other than carbon-dioxide. Methanogens are found in dark basaltic rocks, mid-ocean ridges, and extreme conditions such as hot springs, hydrothermal vents, and more.
To determine: The type of carbon fixation pathway used.
Introduction: The carbon fixation pathway is mostly found in the autotrophs, usually during the process of photosynthesis. The most common method of carbon fixation is the Calvin cycle. The other method of carbon fixation is CAM or Crassulacean acid
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