Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 20, Problem 35EQ
Summary Introduction
To review:
The expected results of electrophoretic mobility shift assay (EMSA) done on the following samples:
Lane 1. 600 base pair (bp) fragment containinga glucocorticoid response element (GRE) and glucocorticoid receptor.
Lane 2. 600 bp segment having a GRE, glucocorticoid receptor, and glucocorticoid hormone.
Lane 3. 600 bp segment with a GRE and CREB (cyclic adenosine monophosphate (cAMP) response element binding protein) protein.
Lane 4. 700 bp segment having a cAMP response element (CRE) and CREB protein.
Lane 5. 700 bp fragment having a CRE along with CREB protein, and hormone which causes phosphorylation of CREB protein.
Lane 6. 700 bp segment with a CRE as well as glucocorticoid receptor, and glucocorticoid hormone.
Introduction:
Electrophoretic mobility shift assay is done to study the interaction of protein and deoxyribonucleic acid (DNA) or the interaction of protein and ribonucleic acid (RNA). This technique is used to find out whether the given protein or the mixture of proteins can bind to a specific DNA or RNA fragment.
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Chapter 20 Solutions
Genetics: Analysis and Principles
Ch. 20.1 - 1. Which of the following may be used as a vector...Ch. 20.1 - The restriction enzymes used in gene-cloning...Ch. 20.1 - 3. Which is the proper order of the following...Ch. 20.1 - 4. The function of reverse transcriptase is...Ch. 20.1 - A collection of recombinant vectors that carry...Ch. 20.2 - Prob. 1COMQCh. 20.2 - Prob. 2COMQCh. 20.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 20.3 - When a dideoxyribonucleotide is incorporated into...Ch. 20.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 20.5 - Which of the following methods use(s) a labeled...Ch. 20.5 - 2. Which of the following methods is used to...Ch. 20.5 - During Western blotting, the primary antibody...Ch. 20.6 - 1. In an EMSA, the binding of a protein to...Ch. 20.6 - The basis for DNase I footprinting is that the...Ch. 20 - Discuss three important advances that have...Ch. 20 - Prob. 2CONQCh. 20 - Write a double-stranded DNA sequence that is 20...Ch. 20 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 20 - 5. Draw the structural feature of a...Ch. 20 - Prob. 1EQCh. 20 - Prob. 2EQCh. 20 - Describe the important features of cloning...Ch. 20 - 4. How does gene cloning produce many copies of a...Ch. 20 - Prob. 5EQCh. 20 - Prob. 6EQCh. 20 - Prob. 7EQCh. 20 - Prob. 8EQCh. 20 - Prob. 9EQCh. 20 - Starting with a sample of RNA that contains the...Ch. 20 - 11. What type of probe is used for real-time PCR?...Ch. 20 - 12. What phase of PCR (exponential, linear, or...Ch. 20 - 13. DNA sequencing can help us to identify...Ch. 20 - A sample of DNA was subjected to automated DNA...Ch. 20 - Prob. 15EQCh. 20 - Prob. 16EQCh. 20 - Prob. 17EQCh. 20 - Prob. 18EQCh. 20 - Prob. 19EQCh. 20 - E20. In Southern, Northern, and Western blotting,...Ch. 20 - What is the purpose of a Northern blotting...Ch. 20 - Prob. 22EQCh. 20 - Prob. 23EQCh. 20 - 23. In the Western blot shown here, proteins were...Ch. 20 - If you wanted to know if a protein was made during...Ch. 20 - Prob. 27EQCh. 20 - Prob. 29EQCh. 20 - Prob. 31EQCh. 20 - 28. Describe the rationale behind the...Ch. 20 - Certain hormones, such as epinephrine, can...Ch. 20 - An electrophoretic mobility shift assay can be...Ch. 20 - Prob. 35EQCh. 20 - Prob. 36EQCh. 20 - Prob. 37EQCh. 20 - Prob. 24EQCh. 20 - Prob. 30EQCh. 20 - Prob. 25EQCh. 20 - Prob. 1QSDC
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