Chapter 20, Problem 19EQ

Summary Introduction

To analyze:

Blotting techniques are used to detect target gene sequence. There are three types of Blotting techniques i.e. Southern, Northern, and western. The Southern, Northern Blotting techniques completely rely on hybridization.

Explain the reason for hybridization in these two techniques. In the process of hybridization which adherent is labeled?

Introduction:

Southern blotting is a technique used to detect the target gene sequence within a mixture of several genes. The method was introduced by Edwin Southern in $1975$. Northern blotting used for the detection of an expression pattern of gene and detection of a copy number of RNA.

The goal of southern blotting andnorthern blotting is to detect if a sample of the genome and RNA contains a complementarity with the specific probe.