Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 20.6, Problem 1COMQ
In an EMSA, the binding of a protein to DNA
a. prevents the DNA from being digested with a restriction enzyme.
b. causes the DNA to migrate more slowly through a gel.
c. causes the DNA to migrate more quickly through a gel.
d. inhibits the expression of any genes within the DNA.
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FLORES DIology Second Semester Review
7. Chi Square Analysis: wild dumpy male x ebony wild female
F1:
All wild wild
F2:
142 wild wild males
52 wild ebony males
46 dumpy wild males
17 dumpy ebony males
151 wild wild females
47 wild ebony females
51 dumpy wild females
8 dumpy ebony females
What pattern of inheritance is observed for body color?
Autosomal recessive
Autosomal dominant
Sex-linked recessive
Sex-linked dominant
What are the genotypes of the parents in the P generation?
a.
XBYdd x XbXbDD
b. Bbdd x bbDd
c. BBdd x bbDD
d. BBX Y x bbXDY
How many flies in the F2 generation would you expect to express the dumpy wild phenotype?
a.
289.125
b. 97
c. 96.375
d. 293
4. This question focuses on entrainment.
a. What is entrainment?
b. What environmental cues are involved in entrainment, and which one is most influential?
c. Why is entrainment necessary?
d. Assuming that a flash of darkness is an effective zeitgeber, what impact on circadian
rhythms would you expect to result from an event such as the 2024 solar eclipse (assume
it was viewed from Carbondale IL, where totality occurred at about 2 pm)? Explain your
reasoning. You may wish to consult this phase response diagram.
Phase Shift (Hours)
Delay Zone
Advance Zone
Dawn
Mid-day
Dusk
Night
Dawn
Time of Light Stimulus
e. Finally, give a real-world example of how knowledge of circadian rhythms and
entrainment has implications for human health and wellbeing or conservation biology.
This example could be from your reading or from things discussed in class.
Generate one question that requires a Punnet Squre to solve the question. Then show how you calculate the possibilities of genotype and phenotype
Chapter 20 Solutions
Genetics: Analysis and Principles
Ch. 20.1 - 1. Which of the following may be used as a vector...Ch. 20.1 - The restriction enzymes used in gene-cloning...Ch. 20.1 - 3. Which is the proper order of the following...Ch. 20.1 - 4. The function of reverse transcriptase is...Ch. 20.1 - A collection of recombinant vectors that carry...Ch. 20.2 - Prob. 1COMQCh. 20.2 - Prob. 2COMQCh. 20.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 20.3 - When a dideoxyribonucleotide is incorporated into...Ch. 20.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 20.5 - Which of the following methods use(s) a labeled...Ch. 20.5 - 2. Which of the following methods is used to...Ch. 20.5 - During Western blotting, the primary antibody...Ch. 20.6 - 1. In an EMSA, the binding of a protein to...Ch. 20.6 - The basis for DNase I footprinting is that the...Ch. 20 - Discuss three important advances that have...Ch. 20 - Prob. 2CONQCh. 20 - Write a double-stranded DNA sequence that is 20...Ch. 20 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 20 - 5. Draw the structural feature of a...Ch. 20 - Prob. 1EQCh. 20 - Prob. 2EQCh. 20 - Describe the important features of cloning...Ch. 20 - 4. How does gene cloning produce many copies of a...Ch. 20 - Prob. 5EQCh. 20 - Prob. 6EQCh. 20 - Prob. 7EQCh. 20 - Prob. 8EQCh. 20 - Prob. 9EQCh. 20 - Starting with a sample of RNA that contains the...Ch. 20 - 11. What type of probe is used for real-time PCR?...Ch. 20 - 12. What phase of PCR (exponential, linear, or...Ch. 20 - 13. DNA sequencing can help us to identify...Ch. 20 - A sample of DNA was subjected to automated DNA...Ch. 20 - Prob. 15EQCh. 20 - Prob. 16EQCh. 20 - Prob. 17EQCh. 20 - Prob. 18EQCh. 20 - Prob. 19EQCh. 20 - E20. In Southern, Northern, and Western blotting,...Ch. 20 - What is the purpose of a Northern blotting...Ch. 20 - Prob. 22EQCh. 20 - Prob. 23EQCh. 20 - 23. In the Western blot shown here, proteins were...Ch. 20 - If you wanted to know if a protein was made during...Ch. 20 - Prob. 27EQCh. 20 - Prob. 29EQCh. 20 - Prob. 31EQCh. 20 - 28. Describe the rationale behind the...Ch. 20 - Certain hormones, such as epinephrine, can...Ch. 20 - An electrophoretic mobility shift assay can be...Ch. 20 - Prob. 35EQCh. 20 - Prob. 36EQCh. 20 - Prob. 37EQCh. 20 - Prob. 24EQCh. 20 - Prob. 30EQCh. 20 - Prob. 25EQCh. 20 - Prob. 1QSDC
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- Briefly state the physical meaning of the electrocapillary equation (Lippman equation).arrow_forwardExplain in a small summary how: What genetic information can be obtained from a Punnet square? What genetic information cannot be determined from a Punnet square? Why might a Punnet Square be beneficial to understanding genetics/inheritance?arrow_forwardIn a small summary write down:arrow_forward
- Not part of a graded assignment, from a past midtermarrow_forwardNoggin mutation: The mouse, one of the phenotypic consequences of Noggin mutationis mispatterning of the spinal cord, in the posterior region of the mouse embryo, suchthat in the hindlimb region the more ventral fates are lost, and the dorsal Pax3 domain isexpanded. (this experiment is not in the lectures).a. Hypothesis for why: What would be your hypothesis for why the ventral fatesare lost and dorsal fates expanded? Include in your answer the words notochord,BMP, SHH and either (or both of) surface ectoderm or lateral plate mesodermarrow_forwardNot part of a graded assignment, from a past midtermarrow_forward
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