Genetics: Analysis and Principles
5th Edition
ISBN: 9780073525341
Author: Robert J. Brooker Professor Dr.
Publisher: McGraw-Hill Education
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Chapter 20, Problem 4CONQ
What is cDNA? In eukaryotes, how does cDNA differ from genomic DNA?
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Chapter 20 Solutions
Genetics: Analysis and Principles
Ch. 20.1 - 1. Which of the following may be used as a vector...Ch. 20.1 - The restriction enzymes used in gene-cloning...Ch. 20.1 - 3. Which is the proper order of the following...Ch. 20.1 - 4. The function of reverse transcriptase is...Ch. 20.1 - A collection of recombinant vectors that carry...Ch. 20.2 - Prob. 1COMQCh. 20.2 - Prob. 2COMQCh. 20.2 - 3. During real-time PCR, the synthesis of PCR...Ch. 20.3 - When a dideoxyribonucleotide is incorporated into...Ch. 20.4 - 1. The purpose of site-directed mutagenesis and...
Ch. 20.5 - Which of the following methods use(s) a labeled...Ch. 20.5 - 2. Which of the following methods is used to...Ch. 20.5 - During Western blotting, the primary antibody...Ch. 20.6 - 1. In an EMSA, the binding of a protein to...Ch. 20.6 - The basis for DNase I footprinting is that the...Ch. 20 - Discuss three important advances that have...Ch. 20 - Prob. 2CONQCh. 20 - Write a double-stranded DNA sequence that is 20...Ch. 20 - What is cDNA? In eukaryotes, how does cDNA differ...Ch. 20 - 5. Draw the structural feature of a...Ch. 20 - Prob. 1EQCh. 20 - Prob. 2EQCh. 20 - Describe the important features of cloning...Ch. 20 - 4. How does gene cloning produce many copies of a...Ch. 20 - Prob. 5EQCh. 20 - Prob. 6EQCh. 20 - Prob. 7EQCh. 20 - Prob. 8EQCh. 20 - Prob. 9EQCh. 20 - Starting with a sample of RNA that contains the...Ch. 20 - 11. What type of probe is used for real-time PCR?...Ch. 20 - 12. What phase of PCR (exponential, linear, or...Ch. 20 - 13. DNA sequencing can help us to identify...Ch. 20 - A sample of DNA was subjected to automated DNA...Ch. 20 - Prob. 15EQCh. 20 - Prob. 16EQCh. 20 - Prob. 17EQCh. 20 - Prob. 18EQCh. 20 - Prob. 19EQCh. 20 - E20. In Southern, Northern, and Western blotting,...Ch. 20 - What is the purpose of a Northern blotting...Ch. 20 - Prob. 22EQCh. 20 - Prob. 23EQCh. 20 - 23. In the Western blot shown here, proteins were...Ch. 20 - If you wanted to know if a protein was made during...Ch. 20 - Prob. 27EQCh. 20 - Prob. 29EQCh. 20 - Prob. 31EQCh. 20 - 28. Describe the rationale behind the...Ch. 20 - Certain hormones, such as epinephrine, can...Ch. 20 - An electrophoretic mobility shift assay can be...Ch. 20 - Prob. 35EQCh. 20 - Prob. 36EQCh. 20 - Prob. 37EQCh. 20 - Prob. 24EQCh. 20 - Prob. 30EQCh. 20 - Prob. 25EQCh. 20 - Prob. 1QSDC
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- 5G JA ATTC 3 3 CTIA A1G5 5 GAAT I I3 3 CTIA AA5 Fig. 5-3: The Eco restriction site (left) would be cleaved at the locations indicated by the arrows. However, a SNP in the position shown in gray (right) would prevent cleavage at this site by EcoRI One of the SNPs in B. rapa is found within the Park14 locus and can be detected by RFLP analysis. The CT polymorphism is found in the intron of the Bra013780 gene found on Chromosome 1. The Park14 allele with the "C" in the SNP has two EcoRI sites and thus is cleaved twice by EcoRI If there is a "T" in that SNP, one of the EcoRI sites is altered, so the Park14 allele with the T in the SNP has only one EcoRI site (Fig. 5-3). Park14 allele with SNP(C) Park14 allele with SNPT) 839 EcoRI 1101 EcoRI 839 EcoRI Fig. 5.4: Schematic restriction maps of the two different Park14 alleles (1316 bp long) of B. rapa. Where on these maps is the CT SNP located? 90 The primers used to amplify the DNA at the Park14 locus (see Fig. 5 and Table 3 of Slankster et…arrow_forwardFrom your previous experiment, you found that this enhancer activates stripe 2 of eve expression. When you sequence this enhancer you find several binding sites for the gap gene, Giant. To test how Giant interacts with eve, you decide to remove all of the Giant binding sites from the eve enhancer. What results do you expect to see with respect to eve expression?arrow_forwardWhat experiment could you do to see if the maternal gene, bicoid, is sufficient to form anterior fates?arrow_forward
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