Chapter 20, Problem 20.25P

Interpretation Introduction

Interpretation:

The weight of DNA in the given aqueous buffer has to be calculated.

Concept Introduction:

Beer-Lambert’s law:

It is the law that is widely used to make quantitative analysis of samples in UV-Spectroscopy. This law establishes a relation between absorbance, concentration and path-length of the sample cell which is known as cuvette in UV-Spectroscopy. The expression of the law is shown here:

$\text{A=}\epsilon \text{C}\text{l}$

$\text{A}\text{=}$ Absorbance (unitless).

$\epsilon$= molar absorptivity (per moles per litre per centimetre, ${\text{M}}^{\text{-1}}{\text{cm}}^{\text{-1}}$).

$\text{C}$= concentration of solute (in moles per litre, $\text{M}$).

$\text{l}$= length of the sample cell or cuvette (in centimetres, $\text{cm}$).