The weight of DNA in the given aqueous buffer has to be calculated. Concept Introduction: Beer-Lambert’s law: It is the law that is widely used to make quantitative analysis of samples in UV-Spectroscopy. This law establishes a relation between absorbance, concentration and path-length of the sample cell which is known as cuvette in UV-Spectroscopy. The expression of the law is shown here: A= ε C l A = Absorbance (unitless). ε = molar absorptivity (per moles per litre per centimetre, M -1 cm -1 ). C = concentration of solute (in moles per litre, M ). l = length of the sample cell or cuvette (in centimetres, cm ).

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Chapter 20, Problem 20.25P

Interpretation Introduction

Interpretation:

The weight of DNA in the given aqueous buffer has to be calculated.

Concept Introduction:

Beer-Lambert’s law:

It is the law that is widely used to make quantitative analysis of samples in UV-Spectroscopy. This law establishes a relation between absorbance, concentration and path-length of the sample cell which is known as cuvette in UV-Spectroscopy. The expression of the law is shown here:

A= ε C l

A = Absorbance (unitless).

ε= molar absorptivity (per moles per litre per centimetre, M-1cm-1).

C= concentration of solute (in moles per litre, M).

l= length of the sample cell or cuvette (in centimetres, cm).

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